Anti-GOLPH3/MIDAS 抗体 (ab236296)
Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GOLPH3/MIDAS antibody
GOLPH3/MIDAS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GOLPH3/MIDAS -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IF, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human GOLPH3/MIDAS aa 1-150.
Database link: Q9H4A6 -
ポジティブ･コントロール
- WB: HeLa, HEK-293 and A549 whole cell lysates. IHC-P: Human prostate cancer and prosate tissues. ICC/IF: HepG2 cells. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purity >95%. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236296の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/20 - 1/200.
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| WB |
1/500 - 1/5000. Predicted molecular weight: 34 kDa.
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| ICC/IF |
1/20 - 1/200.
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| IP |
1/200 - 1/2000.
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| 特記事項 |
|---|
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IHC-P
1/20 - 1/200. |
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WB
1/500 - 1/5000. Predicted molecular weight: 34 kDa. |
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ICC/IF
1/20 - 1/200. |
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IP
1/200 - 1/2000. |
ターゲット情報
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機能
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. -
組織特異性
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. -
配列類似性
Belongs to the GOLPH3/VPS74 family. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. - Information by UniProt
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参照データベース
- Entrez Gene: 64083 Human
- Entrez Gene: 66629 Mouse
- Entrez Gene: 78961 Rat
- Omim: 612207 Human
- SwissProt: Q9H4A6 Human
- SwissProt: Q9CRA5 Mouse
- SwissProt: Q9ERE4 Rat
- Unigene: 408909 Human
see all -
別名
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab236296)
Paraffin-embedded human prostate cancer tissue stained for GOLPH3/MIDAS using ab236296 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-GOLPH3/MIDAS antibody (ab236296)All lanes : Anti-GOLPH3/MIDAS antibody (ab236296) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab236296)Paraffin-embedded human prostate tissue stained for GOLPH3/MIDAS using ab236296 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-GOLPH3/MIDAS antibody (ab236296)4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for GOLPH3/MIDAS(green) using ab236296 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
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Immunoprecipitation - Anti-GOLPH3/MIDAS antibody (ab236296)GOLPH3/MIDAS was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab236296 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HeLa whole cell lysate.
Lane 2: ab236296 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 10 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab236296 は 3 報の論文で使用されています。
- Gao Y et al. Golgi phosphoprotein 3 promotes angiogenesis and sorafenib resistance in hepatocellular carcinoma via upregulating exosomal miR-494-3p. Cancer Cell Int 22:35 (2022). PubMed: 35073936
- Wang K et al. GOLPH3 Promotes Cancer Growth by Interacting With STIP1 and Regulating Telomerase Activity in Pancreatic Ductal Adenocarcinoma. Front Oncol 10:575358 (2020). PubMed: 33134174
- Ouyang J et al. miR-126 targeting GOLPH3 inhibits the epithelial-mesenchymal transition of gastric cancer BGC-823 cells and reduces cell invasion. Eur J Histochem 64:N/A (2020). PubMed: 33131267