Anti-GOLPH3/MIDAS 抗体 (ab113649)
Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GOLPH3/MIDAS antibody
GOLPH3/MIDAS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GOLPH3/MIDAS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal) conjugated to keyhole limpet haemocyanin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113649の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. -
組織特異性
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. -
配列類似性
Belongs to the GOLPH3/VPS74 family. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. - Information by UniProt
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参照データベース
- Entrez Gene: 64083 Human
- Entrez Gene: 66629 Mouse
- Entrez Gene: 78961 Rat
- Omim: 612207 Human
- SwissProt: Q9H4A6 Human
- SwissProt: Q9CRA5 Mouse
- SwissProt: Q9ERE4 Rat
- Unigene: 408909 Human
see all -
別名
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab113649)
ab113649, at 5 µg/ml, staining GOLPH3/MIDAS in Formalin fixed, Paraffin embedded Human prostate tissue by Immunohistochemistry.
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Western blot - Anti-GOLPH3/MIDAS antibody (ab113649)Lane 1 : Anti-GOLPH3/MIDAS antibody (ab113649) at 0.5 µg/ml
Lane 2 : Anti-GOLPH3/MIDAS antibody (ab113649) at 1 µg/ml
All lanes : Rat lung tissue lysate
Predicted band size: 34 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab113649 は 1 報の論文で使用されています。
- El-Maqsoud NMRA et al. GOLPH3 and YB-1 Are Novel Markers Correlating With Poor Prognosis in Prostate Cancer. World J Oncol 6:473-484 (2015). PubMed: 28983350