Anti-GODZ/DHHC-3 抗体 (ab31837)
Key features and details
- Rabbit polyclonal to GODZ/DHHC-3
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-GODZ/DHHC-3 antibody -
製品の詳細
Rabbit polyclonal to GODZ/DHHC-3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Xenopus laevis
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31837の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration. PubMed: 19001095
|
|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 19001095 |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
ターゲット情報
-
機能
Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi. -
配列類似性
Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger. -
ドメイン
The DHHC domain is required for palmitoyltransferase activity. -
翻訳後修飾
Autopalmitoylated. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 51304 Human
- Entrez Gene: 69035 Mouse
- Entrez Gene: 301081 Rat
- SwissProt: Q9NYG2 Human
- SwissProt: Q8R173 Mouse
- Unigene: 61430 Human
- Unigene: 28300 Mouse
-
別名
- DHHC-3 antibody
- GABA-A receptor-associated membrane protein 1 antibody
- GODZ antibody
see all
画像
-
Western blot - Anti-GODZ/DHHC-3 antibody (ab31837)Anti-GODZ/DHHC-3 antibody (ab31837) at 1 µg/ml + Cerebellum (Mouse) Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (10)
ab31837 は 10 報の論文で使用されています。
- Yao H et al. A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions. RSC Chem Biol 2:192-205 (2021). PubMed: 34458782
- Kutchukian C et al. NPC1 regulates the distribution of phosphatidylinositol 4-kinases at Golgi and lysosomal membranes. EMBO J 40:e105990 (2021). PubMed: 34019311
- Mukherjee A et al. Specific Palmitoyltransferases Associate with and Activate the Epithelial Sodium Channel. J Biol Chem 292:4152-4163 (2017). PubMed: 28154191
- Kilpatrick CL et al. Dissociation of Golgi-associated DHHC-type Zinc Finger Protein (GODZ)- and Sertoli Cell Gene with a Zinc Finger Domain-ß (SERZ-ß)-mediated Palmitoylation by Loss of Function Analyses in Knock-out Mice. J Biol Chem 291:27371-27386 (2016). PubMed: 27875292
- Lievens PM et al. ZDHHC3 Tyrosine Phosphorylation Regulates Neural Cell Adhesion Molecule Palmitoylation. Mol Cell Biol 36:2208-25 (2016). WB ; Mouse . PubMed: 27247265
- Lai J & Linder ME Oligomerization of DHHC protein S-acyltransferases. J Biol Chem 288:22862-70 (2013). PubMed: 23793055
- Levy AD et al. Subcellular Golgi localization of stathmin family proteins is promoted by a specific set of DHHC palmitoyl transferases. Mol Biol Cell 22:1930-42 (2011). PubMed: 21471001
- Shmueli A et al. Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity. EMBO J 29:107-19 (2010). WB, ICC/IF ; Human . PubMed: 19927128
- Noritake J et al. Mobile DHHC palmitoylating enzyme mediates activity-sensitive synaptic targeting of PSD-95. J Cell Biol 186:147-60 (2009). WB, ICC/IF ; Rat . PubMed: 19596852
- Tsutsumi R et al. Identification of G protein alpha subunit-palmitoylating enzyme. Mol Cell Biol 29:435-47 (2009). WB, ICC/IF ; Human . PubMed: 19001095