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Anti-GnRH 抗体 (ab16216)

Reviews (1)Q&A (1)References (3)

Key features and details

  • Rabbit polyclonal to GnRH
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-GnRH antibody
    GnRH 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to GnRH

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Fr, IHC-Pmore details

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide:

    pyroEHWSYGLRPG

    , corresponding to amino acids 22-31 of Mouse GnRH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab16216の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.

  • 細胞内局在

    Secreted

  • 参照データベース

    • 別名

      • GnRH associated peptide 1 antibody
      • GNRH1 antibody
      • Gonadotrophin releasing hormone 1 antibody
      • GRH antibody
      • HH12 antibody
      • LHRH antibody
      • LNRH antibody
      • Luliberin I antibody
      • Luteinizing releasing hormone antibody
      • Lutenizing hormome releasing hormone antibody
      • Progonadoliberin 1 antibody
      • Progonadoliberin I antibody
      • Prolactin release inhibiting factor antibody
      see all

    参考文献 (3)

    ab16216 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab16216 は 3 報の論文で使用されています。

    • Chang B  et al. Leptin and inflammatory factors play a synergistic role in the regulation of reproduction in male mice through hypothalamic kisspeptin-mediated energy balance. Reprod Biol Endocrinol 19:12 (2021). PubMed: 33472656
    • Li R  et al. An Antagonistic Peptide of Gpr1 Ameliorates LPS-Induced Depression through the Hypothalamic-Pituitary-Ovarian Axis. Biomolecules 11:N/A (2021). PubMed: 34207497
    • Wang X  et al. The effect of swimming exercise and diet on the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-?B-GnRH expression. Aging (Albany NY) 12:11085-11099 (2020). PubMed: 32518216

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-GnRH antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Recombinant protein (mixed with LbT2 cells 10ug lysate)
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Loading amount
    0.054 µg
    Specification
    mixed with LbT2 cells 10ug lysate
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Lilach Polinsky

    Verified customer

    投稿 Oct 10 2018

    Question

    Dear technical support: The customer would like to perform ELISA in chicken samples. He would like to detect GnRH level in chicken brain samples. However, there is no suitable antibody for him. Datasheet of ab5617 shows immunogene: Synthetic peptide: EHWSYGLRPG (Human). He would like to know the homology of this peptide across chicken. Cold you please help him to check the sequence homology between human across chicken? Thank you very much for your assistance.

    Read More
    Answer

    Thank you for contacting us. Both the ab5617 and ab16216 are raised against an immunogen synthetic peptide of HWSYGLRPG corresponding to amino acids 22-31 of GnRH. Comparing this to the corresponding sequence of Chicken GnRH (Swiss-Prot number P37042) reveals 8 of the 9 amino acids of the immunogen match exactly. We would therefore expect these antibodies to detect chicken GnRH. However, neither of these antibodies have been tested against chicken or with ELISA. Therefore, I can offer a discount off a future purchase if you buy either ab5617 or ab16216 now, test it in chicken with the ELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test either ab5617 or ab16216 in chicken with the ELISA. I will then send a discount code. This code must be issued before purchasing ab5617 or ab16216 so please wait for my reply before ordering. 2. Purchase either ab5617 or ab16216 either by phone, fax, or online (www.abcam.com). 3. Test it in chicken with the ELISA. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if either ab5617 or ab16216 turns out to be unsuitable for chicken with the ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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