Anti-GNPAT/DAPAT 抗体 (ab75060)
Key features and details
- Rabbit polyclonal to GNPAT/DAPAT
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GNPAT/DAPAT antibody -
製品の詳細
Rabbit polyclonal to GNPAT/DAPAT -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab75060 was purified using an epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75060の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 77 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 77 kDa. |
ターゲット情報
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パスウェイ
Membrane lipid metabolism; glycerophospholipid metabolism. -
関連疾患
Defects in GNPAT are the cause of rhizomelic chondrodysplasia punctata type 2 (RCDP2) [MIM:222765]. RDCP2 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation. -
配列類似性
Belongs to the GPAT/DAPAT family. -
ドメイン
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
細胞内局在
Peroxisome membrane. Exclusively localized to the lumenal side of the peroxisomal membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8443 Human
- Entrez Gene: 14712 Mouse
- Omim: 602744 Human
- SwissProt: O15228 Human
- SwissProt: P98192 Mouse
- Unigene: 498028 Human
- Unigene: 29114 Mouse
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別名
- Acyl CoA dihydroxyacetonephosphateacyltransferase antibody
- Acyl-CoA:dihydroxyacetonephosphateacyltransferase antibody
- DAP AT antibody
see all
画像
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Western blot - Anti-GNPAT/DAPAT antibody (ab75060)All lanes : Anti-GNPAT/DAPAT antibody (ab75060) at 1/500 dilution
Lane 1 : extracts from HT-29 cells
Lane 2 : extracts from RAW264.7cells
Lane 3 : extracts from HT-29 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab75060 は 4 報の論文で使用されています。
- Li C et al. CSE triggers ferroptosis via SIRT4-mediated GNPAT deacetylation in the pathogenesis of COPD. Respir Res 24:301 (2023). PubMed: 38041059
- Hossain MS et al. Plasmalogens, the Vinyl Ether-Linked Glycerophospholipids, Enhance Learning and Memory by Regulating Brain-Derived Neurotrophic Factor. Front Cell Dev Biol 10:828282 (2022). PubMed: 35223852
- Hossain MS et al. Reduction of Ether-Type Glycerophospholipids, Plasmalogens, by NF-?B Signal Leading to Microglial Activation. J Neurosci 37:4074-4092 (2017). PubMed: 28292831
- Hossain MS et al. Neuronal Orphan G-Protein Coupled Receptor Proteins Mediate Plasmalogens-Induced Activation of ERK and Akt Signaling. PLoS One 11:e0150846 (2016). WB . PubMed: 26934370