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Anti-GNL1 抗体 (ab245634)

Submit a review Submit a question References (1)
Western blot - Anti-GNL1 antibody (ab245634)
  • Immunoprecipitation - Anti-GNL1 antibody (ab245634)

Key features and details

  • Rabbit polyclonal to GNL1
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-GNL1 antibody

  • 製品の詳細

    Rabbit polyclonal to GNL1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chimpanzee, Orangutan

  • 免疫原

    Synthetic peptide within Human GNL1 aa 50-100. The exact sequence is proprietary. NP_005266.2
    Database link: P36915

  • ポジティブ･コントロール

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    ab245634 was affinity purified using an epitope specific to GNL1 immobilized on solid support.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245634の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at 5-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
特記事項
IP
Use at 5-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 69 kDa.

ターゲット情報

  • 機能

    Possible regulatory or functional link with the histocompatibility cluster.

  • 配列類似性

    Belongs to the MMR1/HSR1 GTP-binding protein family.
    Contains 1 G (guanine nucleotide-binding) domain.

  • ドメイン

    In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Gnl1 antibody
    • GNL1_HUMAN antibody
    • GTP binding protein HSR1 antibody
    • GTP-binding protein HSR1 antibody
    • Guanine nucleotide binding protein like 1 antibody
    • Guanine nucleotide-binding protein-like 1 antibody
    • HSR1 antibody
    • HSR1 GTP binding protein antibody
    see all

画像

  • Western blot - Anti-GNL1 antibody (ab245634)
    Western blot - Anti-GNL1 antibody (ab245634)
    All lanes : Anti-GNL1 antibody (ab245634) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 69 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-GNL1 antibody (ab245634)
    Immunoprecipitation - Anti-GNL1 antibody (ab245634)

    GNL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245634 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245634 at 0.4 µg/ml.

    Lane 1: ab245634 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab245634 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab245634 は 1 報の論文で使用されています。

  • Turcotte MA  et al. Guanine Nucleotide-Binding Protein-Like 1 (GNL1) binds RNA G-quadruplex structures in genes associated with Parkinson's disease. RNA Biol 18:1339-1353 (2021). PubMed: 33305682

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