Anti-GNAI3 抗体 (ab154024)
Key features and details
- Rabbit polyclonal to GNAI3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GNAI3 antibody
GNAI3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GNAI3 -
由来種
Rabbit -
特異性
Immunogen shares high homology with human GNAI1 and GNAI2, and with other proteins of the Galpha family and therefore cross-reactivity may occur.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
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免疫原
Recombinant fragment, corresponding to a region within amino acids 68-354 of Human GNAI3 (Uniprot ID: P08754).
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ポジティブ･コントロール
- 293T, H1299, HeLaS3, HepG2 cells; A431, MOLT4, Raji and Mouse brain whole cell lysates; Human SAS xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154024の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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| IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. -
配列類似性
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
細胞内局在
Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody. - Information by UniProt
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参照データベース
- Entrez Gene: 374097 Chicken
- Entrez Gene: 539521 Cow
- Entrez Gene: 2773 Human
- Entrez Gene: 14679 Mouse
- Entrez Gene: 100144421 Pig
- Entrez Gene: 25643 Rat
- Entrez Gene: 379236 Xenopus laevis
- Entrez Gene: 496962 Xenopus tropicalis
see all -
別名
- 87U6 antibody
- FLJ26559 antibody
- G protein alpha inhibiting 3 antibody
see all
画像
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Western blot - Anti-GNAI3 antibody (ab154024)All lanes : Anti-GNAI3 antibody (ab154024) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lane 3 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
7.5% SDS PAGE -
Western blot - Anti-GNAI3 antibody (ab154024)Anti-GNAI3 antibody (ab154024) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 41 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAI3 antibody (ab154024)Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling GNAI3 with ab154024 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154024 は 3 報の論文で使用されています。
- Wang X et al. CCDC88A/GIV promotes HBV replication and progeny secretion via enhancing endosomal trafficking and blocking autophagic degradation. Autophagy 18:357-374 (2022). PubMed: 34190023
- Meng L et al. Mir24-2-5p suppresses the osteogenic differentiation with Gnai3 inhibition presenting a direct target via inactivating JNK-p38 MAPK signaling axis. Int J Biol Sci 17:4238-4253 (2021). PubMed: 34803495
- Zhang Y et al. Guanine and nucleotide binding protein 3 promotes odonto/osteogenic differentiation of apical papilla stem cells via JNK and ERK signaling pathways. Int J Mol Med 43:382-392 (2019). PubMed: 30431055