AB137050

Anti-GNAI2 抗体 [EPR9468]

Anti-GNAI2 antibody [EPR9468]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal GNAI2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 5 publications.

別名を表示する

GNAI2B, GNAI2, Guanine nucleotide-binding protein G(i) subunit alpha-2, Adenylate cyclase-inhibiting G alpha protein

2 Images

Flow Cytometry (Intracellular) - Anti-GNAI2 antibody [EPR9468] (AB137050)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GNAI2 antibody [EPR9468] (AB137050)

Intracellular flow cytometric analysis of permeabilized HeLa cells labelling GNAI2 with ab137050 at dilution 1/10 (red) or a Rabbit IgG negative control (green).

Unknown

Western blot - Anti-GNAI2 antibody [EPR9468] (AB137050)

All lanes:

Western blot - Anti-GNAI2 antibody [EPR9468] (ab137050) at 1/1000 dilution

Lane 1:

HL60 cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

U-87 MG cell lysate at 10 µg

Lane 6:

C6 cell lysate at 10 µg

Lane 7:

RAW 264.7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 40 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR9468

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GNAI2 also known as Gi2 alpha-subunit is a G protein subunit encoded by the GNAI2 gene. It has a molecular weight of approximately 40 kDa. It influences intracellular signaling pathways by inhibiting adenylyl cyclase and decreasing the levels of cyclic AMP (cAMP) within the cell. GNAI2 is found in a variety of tissues with high expression in the brain heart and lymphoid tissues. This wide expression pattern shows its importance in different physiological processes across various organs.

Biological function summary

GNAI2 functions as part of heterotrimeric G protein complexes. It couples with receptors on the cell surface transmitting signals from activated receptors to downstream effectors. GNAI2 binds with GDP in its inactive state and exchanges it for GTP upon receptor activation which causes the dissociation of the G protein into G alpha and G beta-gamma subunits continuing the signaling cascade. GNAI2 contributes to the modulation of processes such as neurotransmission immune responses and cell proliferation.

Pathways

GNAI2 plays an important role in the MAPK/ERK pathway and the PI3K/Akt signaling pathway. It interacts with various receptors and proteins to regulate cellular responses to external stimuli. For instance through the MAPK/ERK pathway GNAI2 influences gene expression and cell division while in the PI3K/Akt pathway it plays a role in cell survival and apoptosis. Its interaction with proteins like G-beta and G-gamma subunits emphasizes its integrative function in coordinating signal transduction.

GNAI2 has links to certain types of cancer and autoimmune diseases. Mutations or dysregulations in GNAI2 expression can influence cancer progression through abnormal cell signaling pathways. Additionally in autoimmune disorders aberrations in GNAI2 signaling can disrupt immune responses contributing to the pathology of diseases like systemic lupus erythematosus. GNAI2 interacts with proteins like adenylyl cyclase and specific receptor proteins which are important for maintaining normal cellular signaling and their dysregulation can lead to disease.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 38505600, PubMed : 29925945, PubMed : 35637350, PubMed : 35365641). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 12359238). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 29925945). Examples of interacting GPCRs include the adenosine A1 receptor/ADORA1, CNR1, and FPR2 (PubMed : 29925945, PubMed : 35637350, PubMed : 35365641). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 12359238). Signaling is mediated via effector proteins, such as adenylate cyclase : inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels. Plays an important role in the activation of the transcription factor NFAT in endothelial cells to promote angiogenesis (PubMed : 38505600). The G(i) proteins are involved in hormonal regulation of adenylate cyclase : they inhibit the cyclase in response to beta-adrenergic stimuli. Plays an essential role for neutrophil recruitment in the context of acute inflammation suggesting a linked function of both GNAI2 in neutrophils and endothelial cells for polymorphonuclear neutrophils transmigration (By similarity).. Isoform sGi2. Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool.

See full target information GNAI2

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7226 PubMed40764514

2025

14-3-3 binding maintains the Parkinson's associated kinase LRRK2 in an inactive state.

Applications

Unspecified application

Species

Unspecified reactive species

Juliana A Martinez Fiesco,Alexandra Beilina,Astrid Alvarez de la Cruz,Ning Li,Riley D Metcalfe,Mark R Cookson,Ping Zhang

The Journal of biological chemistry 299:105088 PubMed37495107

2023

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Nůsková,Fabiola Garcia Cortizo,Lena Sophie Schwenker,Timo Sachsenheimer,Egor E Diakonov,Marcel Tiebe,Martin Schneider,Jasmin Lohbeck,Carissa Reid,Annette Kopp-Schneider,Dominic Helm,Britta Brügger,Aubry K Miller,Aurelio A Teleman

Journal of microbiology and biotechnology 32:126-140 PubMed34750284

2021

Global Proteomic Analysis of Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells via Connective Tissue Growth Factor Treatment under Chemically Defined Feeder-Free Culture Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hye Seo,Young-Joo Jeon

Nature communications 12:4590 PubMed34321466

2021

Stearic acid blunts growth-factor signaling via oleoylation of GNAI proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Nůsková,Marina V Serebryakova,Anna Ferrer-Caelles,Timo Sachsenheimer,Christian Lüchtenborg,Aubry K Miller,Britta Brügger,Larisa V Kordyukova,Aurelio A Teleman

Journal of hepatology 70:722-734 PubMed30582979

2018

Hepatocyte-specific suppression of microRNA-221-3p mitigates liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Chieh Tsay,Qinggong Yuan,Asha Balakrishnan,Marina Kaiser,Selina Möbus,Emilia Kozdrowska,Marwa Farid,Pia-Katharina Tegtmeyer,Katharina Borst,Florian W R Vondran,Ulrich Kalinke,Andreas Kispert,Michael P Manns,Michael Ott,Amar Deep Sharma

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GNAI2 抗体 [EPR9468]

Anti-GNAI2 antibody [EPR9468]

Flow Cytometry (Intracellular) - Anti-GNAI2 antibody [EPR9468] (AB137050)

Western blot - Anti-GNAI2 antibody [EPR9468] (AB137050)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

Abcam product promise