Anti-GMDS 抗体 (ab238613)
Key features and details
- Rabbit polyclonal to GMDS
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GMDS antibody
GMDS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GMDS -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chinese hamster
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免疫原
Recombinant fragment corresponding to Human GMDS aa 200-350.
Database link: O60547 -
ポジティブ･コントロール
- IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purity >95% -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab238613の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/500.
|
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| ICC/IF |
1/50 - 1/200.
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| 特記事項 |
|---|
|
IHC-P
1/200 - 1/500. |
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ICC/IF
1/50 - 1/200. |
ターゲット情報
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機能
Conversion of GDP-D-mannose to GDP-4-keto-6-D-deoxymannose. -
パスウェイ
Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2. -
配列類似性
Belongs to the GDP-mannose 4,6-dehydratase family. - Information by UniProt
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参照データベース
- Entrez Gene: 2762 Human
- Entrez Gene: 218138 Mouse
- Omim: 602884 Human
- SwissProt: Q8K3X3 Chinese hamster
- SwissProt: O60547 Human
- SwissProt: Q8K0C9 Mouse
- Unigene: 144496 Human
- Unigene: 660919 Human
see all -
別名
- GDP D mannose dehydratase antibody
- GDP mannose 4 6 dehydratase antibody
- GDP-D-mannose dehydratase antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMDS antibody (ab238613)
Paraffin-embedded human breast cancer tissue stained for GMDS using ab238613 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-GMDS antibody (ab238613)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GMDS (Green) using ab238613 at 1/133 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counterstain was DAPI (Blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab238613 は論文での使用が確認できていません。