Anti-GM-CSF 抗体 [EPR25991-223] (BSA and Azide free) (ab300496)
![Western blot - Anti-GM-CSF antibody [EPR25991-223] (BSA and Azide free) (ab300496)](https://www.abcam.co.jp/ps/products/300/ab300496/Images/AB300496-486147-anti-CSF2-antibody-epr25991-223-western-blot.jpg "Western blot - Anti-GM-CSF antibody [EPR25991-223] (BSA and Azide free) (ab300496)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25991-223] to GM-CSF - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GM-CSF antibody [EPR25991-223] (BSA and Azide free)
GM-CSF 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25991-223] to GM-CSF - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: ICC/IF,IHC-Fr or IHC-P -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HuT-78 and Jurkat treated with 80nM TPA and 30µM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours whole cell lysate. IP: HuT-78 treated with 80nM TPA and 30µM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours cell lysates.
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特記事項
AB300496 is the carrier-free version of AB300495.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25991-223 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300496の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 16 kDa.
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| IP |
1/30.
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| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 16 kDa. |
|
IP
1/30. |
ターゲット情報
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機能
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. -
配列類似性
Belongs to the GM-CSF family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 1437 Human
- Omim: 138960 Human
- SwissProt: P04141 Human
- Unigene: 1349 Human
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別名
- Colony stimulating factor 2 (granulocyte-macrophage) antibody
- Colony Stimulating Factor 2 antibody
- Colony stimulating factor antibody
see all
画像
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Western blot - Anti-GM-CSF antibody [EPR25991-223] (BSA and Azide free) (ab300496)All lanes : Anti-GM-CSF antibody [EPR25991-223] (ab300495) at 1/1000 dilution
Lane 1 : Untreated HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
Lane 2 : HuT-78 treated with 80nM TPA and 30µM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours whole cell lysate
Lane 3 : Untreated Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 4 : Jurkat treated with 80nM TPA and 30µM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 16 kDa
Observed band size: 15,18,22 kDa why is the actual band size different from the predicted?This data was developed using ab300495, the same antibody clone in a different buffer formulation.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 14512323).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lane 1-2: 15 seconds
Lane 3-4: 37 seconds -
![Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (BSA and Azide free) (ab300496)](https://www.abcam.co.jp/ps/products/300/ab300496/Images/AB300496-1-anti-CSF2-antibody-epr25991-223-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GM-CSF antibody [EPR25991-223] (BSA and Azide free) (ab300496)GM-CSF was immunoprecipitated from 10 µg of HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30μM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours whole cell lysate with ab300495 at 1/30 dilution. VeriBlot for IP secondary antibody(HRP) (ab131366) at 1/5000 dilution was used as a secondary antibody.
Lane 1: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) treated with 80nM TPA and 30μM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours whole cell lysate 10 µg (Input).
Lane2: ab300495 IP in HuT-78 treated with 80nM TPA and 30μM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours cell lysates.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300495 in HuT-78 treated with 80nM TPA and 30μM ionomycin for 5 hours, then with 300ng/ml BFA added for 4 hours cell lysates.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exsposure time: 75 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 14512323).
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![Anti-CSF2 antibody [EPR25991-223] (BSA and Azide free) (AB300496)](https://www.abcam.co.jp/ps/products/300/ab300496/Images/AB300496-3-benefitsofrecombinantantibodies.jpg)
Anti-CSF2 antibody [EPR25991-223] (BSA and Azide free) (AB300496)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300496 は論文での使用が確認できていません。
