Anti-Glycophorin C/GPC 抗体 (ab175257)
Key features and details
- Rabbit polyclonal to Glycophorin C/GPC
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Glycophorin C/GPC antibody
Glycophorin C/GPC 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Glycophorin C/GPC -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human Glycophorin C/GPC aa 1-128.
Sequence:MWSTRSPNSTAWPLSLEPDPGMASASTTMHTTTIAEPDPGMSGWPDGRME TSTPTIMDIVVIAGVIAAVAIVLVSLLFVMLRYMYRHKGTYHTNEAKGTE FAESADAALQGDPALQDAGDSSRKEYFI
Database link: P04921 -
ポジティブ･コントロール
- TF-1 and K562 cell lysate and Red blood cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175257の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/500 - 1/2000. Predicted molecular weight: 14 kDa.
|
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/500 - 1/2000. Predicted molecular weight: 14 kDa. |
ターゲット情報
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機能
This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells. -
組織特異性
Glycophorin-C is expressed in erythrocytes. Glycophorin-D is ubiquitous. -
配列類似性
Belongs to the glycophorin-C family. -
細胞内局在
Cell membrane. Linked to the membrane via band 4.1. - Information by UniProt
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参照データベース
- Entrez Gene: 2995 Human
- Omim: 110750 Human
- SwissProt: P04921 Human
- Unigene: 59138 Human
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別名
- CD236 antibody
- CD236R antibody
- GE antibody
see all
画像
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Western blot - Anti-Glycophorin C/GPC antibody (ab175257)All lanes : Anti-Glycophorin C/GPC antibody (ab175257) at 1/500 dilution
Lane 1 : TF-1 cell line lysate
Lane 2 : K562 cell line lysate
Lane 3 : Red blood cells
Predicted band size: 14 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin C/GPC antibody (ab175257)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophageal cancer tissue labelling GYPC with ab175257 at 1/200. Magnification: 200x. -
Immunocytochemistry/ Immunofluorescence - Anti-Glycophorin C/GPC antibody (ab175257)Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab175257. Blue DAPI for nuclear staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab175257 は 1 報の論文で使用されています。
- Adderley JD et al. Analysis of erythrocyte signalling pathways during Plasmodium falciparum infection identifies targets for host-directed antimalarial intervention. Nat Commun 11:4015 (2020). PubMed: 32782246