Anti-Glycophorin A 抗体 [YTH89.1] (ab33386)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [YTH89.1] (ab33386)](https://www.abcam.co.jp/ps/products/33/ab33386/Images/ab33386.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [YTH89.1] (ab33386)")
Key features and details
- Rat monoclonal [YTH89.1] to Glycophorin A
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-Glycophorin A antibody [YTH89.1]
Glycophorin A 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [YTH89.1] to Glycophorin A -
由来種
Rat -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Human Glycophorin A.
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ポジティブ･コントロール
- IHC-P: Human hypophysis.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
YTH89.1 -
ミエローマ
Y3/Ag1.2.3 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33386の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. |
| 特記事項 |
|---|
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IHC-P
1/100 - 1/1000. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. |
ターゲット情報
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機能
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). -
配列類似性
Belongs to the glycophorin A family. -
翻訳後修飾
The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific). -
細胞内局在
Cell membrane. Appears to be colocalized with SLC4A1. - Information by UniProt
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参照データベース
- Entrez Gene: 2993 Human
- Omim: 111300 Human
- SwissProt: P02724 Human
- Unigene: 434973 Human
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別名
- AI853584 antibody
- Blood group--MN locus antibody
- CD_antigen=CD235a antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [YTH89.1] (ab33386)ab33386 staining erythrocytes in sinusoids of human hypophysis by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab33386 は 3 報の論文で使用されています。
- Furuya T et al. Reticulocytes from cryopreserved erythroblasts support Plasmodium vivax infection in vitro. Parasitol Int 63:278-84 (2014). PubMed: 24291603
- Jokiranta TS & Meri S Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement. J Immunol 151:2124-31 (1993). PubMed: 7688394
- Outram S et al. Erythromyeloid lineage fidelity is conserved in erythroleukaemia. Leuk Res 12:651-7 (1988). PubMed: 3184981
