Anti-Glycophorin A + B 抗体 [HIR2] (ab15009)
Key features and details
- Mouse monoclonal [HIR2] to Glycophorin A + B
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-Glycophorin A + B antibody [HIR2]
Glycophorin A + B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HIR2] to Glycophorin A + B -
由来種
Mouse -
特異性
The antibody recognizes N-terminal, homologous portion of glycophorins A (GPA) and B (GPB), (strongly to GPA, and weakly to GPB). The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).
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ポジティブ・コントロール
- FACS: peripheral blood leukocytes
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
HIR2 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15009の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P | (1) |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). -
細胞内局在
Type I membrane protein. -
参照データベース
- Entrez Gene: 2993 Human
- Entrez Gene: 2994 Human
- SwissProt: P02724 Human
- SwissProt: P06028 Human
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別名
- CD235a antibody
- CD235b antibody
- Erythroid lineage specific membrane sialoglycoprotein antibody
see all
画像
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ab15009 staining Human normal lung tissue. Staining is localised to cellular membranes. Left panel: with primary antibody at 1 µg/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus, at RT. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins followed by blocking with Dako Protein block for 10 mins (containing casein 0.25% in PBS) then incubated with primary antibody for 20 mins and detected with Dako Envision Flex amplification kit for 30 mins. Colorimetric detection was completed with DAB for 5 mins. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Overlay histogram showing K562 cells stained with ab15009 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab15009, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Flow cytometry analysis of human peripheral blood cells labelling Glycophorin A + B with ab15009. A APC-conjugated goat anti-mouse IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab15009 は 5 報の論文で使用されています。
- Soderblom EJ et al. Proteomic analysis of ERK1/2-mediated human sickle red blood cell membrane protein phosphorylation. Clin Proteomics 10:1 (2013). WB, IP ; Human . PubMed: 23286773
- Zhang X et al. Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming. Oncogene : (2012). PubMed: 22777357
- Thorogate R et al. A novel fluorescence-based method in forensic science for the detection of blood in situ. Forensic Sci Int Genet 2:363-71 (2008). ICC/IF ; Human . PubMed: 19083849
- Rogers CE et al. Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship with burst-forming units-erythroid. Exp Hematol 24:597-604 (1996). PubMed: 8605964
- Nakahata T & Okumura N Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma 13:401-9 (1994). PubMed: 8069185