JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB228615

Anti-Glycerol kinase 抗体

Anti-Glycerol kinase antibody

  • JP Deleterious:医薬用外劇物

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal Glycerol kinase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glycerol kinase.

別名を表示する

Glycerol kinase, Glycerokinase, ATP:glycerol 3-phosphotransferase, GK

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Glycerol kinase antibody (AB228615)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glycerol kinase antibody (AB228615)

Ice-cold methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Glycerol kinase (green) using ab228615 at 1/500 dilution in ICC/IF.

Nuclear counter stain : Hoechst 33342 (blue).

Western blot - Anti-Glycerol kinase antibody (AB228615)
  • WB

Supplier Data

Western blot - Anti-Glycerol kinase antibody (AB228615)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Glycerol kinase antibody (ab228615) at 1/1000 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 61 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human Glycerol kinase. The exact immunogen used to generate this antibody is proprietary information.

P32189

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glycerol kinase also known as GK or Gyk acts as a catalyst in the phosphorylation of glycerol to glycerol-3-phosphate. This enzymatic activity is important for the utilization of glycerol in metabolic processes. Glycerol kinase is expressed in tissues such as liver kidney and adipose tissue. Its molecular weight varies depending on the species but in humans it is around 60 kDa when analyzed using methods like glycerol SDS PAGE. In human cells like HEK293T reliable detection of glycerol kinase is possible due to its considerable expression.
Biological function summary

The main role of glycerol kinase relates to its function in lipid metabolism and energy homeostasis. It exists as a part of a biochemical pathway but does not typically form a complex with other proteins. Glycerol kinase enables cells to utilize glycerol as an energy source by converting it into glycerol-3-phosphate which feeds into further metabolic pathways. This conversion is essential for the balance of energy supply and the production of triglycerides and phospholipids.

Pathways

Metabolism and energy production heavily rely on the function of glycerol kinase. This enzyme acts within the glycerolipid metabolism and gluconeogenesis pathways. In these pathways it is connected to other enzymes like glycerol-3-phosphate dehydrogenase which further processes the glycerol-3-phosphate produced by glycerol kinase. The enzyme's availability affects the rate of these pathways and in turn influences lipid and glucose metabolism in the body.

Glycerol kinase deficiency leads to complex metabolic syndromes such as hyperglycerolemia and congenital adrenal hyperplasia. Hyperglycerolemia results from excess glycerol in the blood due to impaired conversion by the enzyme. In congenital adrenal hyperplasia alterations in metabolic function relate to glycerol kinase’s impact on stress response and energy balance. Disorders related to glycerol kinase sometimes involve interactions with other proteins and enzymes involved in broader metabolic processes affecting overall metabolism and physiology.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Kinase that plays a key role in glycerol metabolism, catalyzing its phosphorylation to produce sn-glycerol 3-phosphate. Sn-glycerol 3-phosphate is a crucial intermediate in various metabolic pathways, such as the synthesis of glycerolipids and triglycerides, glycogenesis, glycolysis and gluconeogenesis.
See full target information Glycerol kinase
style
left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401311 PubMed39418169

2024

Glycerol Kinase Drives Hepatic de novo Lipogenesis and Triglyceride Synthesis in Nonalcoholic Fatty Liver by Activating SREBP-1c Transcription, Upregulating DGAT1/2 Expression, and Promoting Glycerol Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyu Ouyang,Shu Zhuo,Mengmei Yang,Tengfei Zhu,Shuting Yu,Yu Li,Hao Ying,Yingying Le

Neurology(R) neuroimmunology & neuroinflammation 10: PubMed37591767

2023

Electrolyte Imbalance in Anti-LGI1 Encephalitis: It Is Not All in Your Head.

Applications

Unspecified application

Species

Unspecified reactive species

Avi Gadoth,Michal Nisnboym,Yifat Alcalay,Asia Zubkov,Idit Schwartz,Doron Schwartz,Marana Abboud,Tamar Rubinek,Ofer Yossepowitch,Talia Weinstein

Biology 10: PubMed34356519

2021

Downregulation of Ribosomal Contents and Kinase Activities Is Associated with the Inhibitive Effect on the Growth of Group B Streptococcus Induced by Placental Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Gao,Yunhui Tang,Xinyi Sun,Qiujing Chen,Yiqian Peng,Catherine Jia-Yun Tsai,Qi Chen
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB193521

Anti-Glycerol kinase antibody

Western blot - Anti-Glycerol kinase antibody (AB193521)

  • 0
  • 0
Primary Antibodies

AB126599

Anti-Glycerol kinase antibody [EPR6567]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-Glycerol kinase antibody [EPR6567] (AB126599)

  • 5
  • 1

組合せ製品

Assay Kits

AB65620

Glycogen Assay Kit

Functional Studies - Glycogen Assay Kit (AB65620)

  • 0
  • 0
Primary Antibodies

AB209211

Anti-Catalase antibody [EPR20198] - Peroxisome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (AB209211)

  • 5
  • 3
Primary Antibodies

AB52649

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649)

  • 5
  • 39
Primary Antibodies

AB211542

Anti-CDKN2A/p16INK4a antibody [EPR20418]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p16INK4a antibody [EPR20418] (AB211542)

  • 5
  • 8
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com