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AB313871

Anti-Glycerol 3 Phosphate Dehydrogenase 抗体 [EPR25996-4]

Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • 詳細を見る

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Rabbit Recombinant Monoclonal GPDA antibody. Suitable for WB, IP, IHC-P and reacts with Human, Mouse, Rat samples.

別名を表示する

GPD-C, GPDH-C, GPD1

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on human kidney tubules (PMID : 34098990). The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on human liver. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunoprecipitation - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • IP

Supplier Data

Immunoprecipitation - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Glycerol 3 Phosphate Dehydrogenase was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab313871 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313871 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 2 : ab313871 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313871 in HepG2 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds

All lanes:

Immunoprecipitation - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (ab313871) at 1/30 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 15s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on rat kidney tubules. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on mouse liver. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on mouse kidney tubules (PMID : 6782104). The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic and nuclear staining on rat liver. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • WB

Supplier Data

Western blot - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : spleen. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : 1 seconds

All lanes:

Western blot - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (ab313871) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Lane 6:

Rat skeletal muscle tissue lysate at 20 µg

Lane 7:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 35 kDa

false

Exposure time: 1s

Western blot - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • WB

Supplier Data

Western blot - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : MCF7 and SK-BR-3. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (ab313871) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human skeletal muscle cytoplasma tissue lysate at 20 µg

Lane 5:

human liver tissue lysate at 20 µg

Lane 6:

Mouse liver tissue lysate at 20 µg

Lane 7:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa

false

Exposure time: 15s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human spleen. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] (AB313871)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab313871 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse spleen. The section was incubated with ab313871 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR25996-4

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse, Rat

アプリケーション

IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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下記製品にもご興味をお持ちいただけるかもしれません:

AB174094

Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric)

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こちらの製品も推奨する理由

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glycerol 3-Phosphate Dehydrogenase (G3PDH) also known as G3P dehydrogenase and glycerol-3-phosphate dehydrogenase plays a mechanical role in the reversible redox conversion of dihydroxyacetone phosphate (DHAP) to glycerol 3-phosphate. The enzyme typically weighs around 40-50 kDa although the exact mass depends on isoform. G3PDH is expressed in various tissues with significant presence in the brain liver and skeletal muscle reflecting its involvement in energy metabolism. The mitochondrial and cytosolic forms add to its versatility in cellular compartments.
Biological function summary

This enzyme is critical for lipid and carbohydrate metabolism engaging extensively in energy homeostasis. G3PDH exists as part of a complex to facilitate its role in mitochondrial electron transport. It acts as a shuttle transferring electrons from cytosolic NADH into the mitochondria. This electron transport is essential for synthesizing ATP thereby linking metabolic pathways within cells.

Pathways

Glycerol-3-phosphate dehydrogenase participates actively in the glycerol phosphate shuttle and lipid biosynthesis pathways. It pairs functionally with its partner the mitochondrial glycerol 3-phosphate dehydrogenase (mG3PDH) to support efficient energy production and lipid storage. Additionally this enzyme relates closely to proteins like NADH dehydrogenase playing a role in electron transport chains in mitochondria critical for bioenergetics.

G3PDH has connections to metabolic conditions such as obesity and diabetes. Impaired function of this enzyme can lead to disruptions in metabolic pathways which can contribute to these diseases. It also holds significance in cancer metabolism with altered expression of G3PDH seen in various tumors. The enzyme's relation to proteins like mG3PDH highlights its role in metabolic syndrome and associated pathologies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Has glycerol-3-phosphate dehydrogenase activity.
See full target information Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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関連製品

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Alternative Version
Primary Antibodies

AB313872

Anti-Glycerol 3 Phosphate Dehydrogenase antibody [EPR25996-4] - BSA and Azide free

primary-antibodies

glycerol-3-phosphate-dehydrogenase-antibody-epr25996-4-bsa-and-azide-free-ab313872

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(0 reviews)

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