JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108524

Anti-Glutathione S Transferase mu 抗体 [EPR1936(2)]

Anti-Glutathione S Transferase mu antibody [EPR1936(2)]

4

(3 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal GSTM1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

別名を表示する

GST1, GSTM1, Glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4

4 Images
Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)
  • WB

Lab

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution

All lanes:

PC-12 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Exposure time: 30s

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)
  • WB

Unknown

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)

All lanes:

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Predicted band size: 26 kDa

false

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)
  • WB

Lab

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

SH-SY5Y whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)
  • WB

Lab

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (AB108524)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution

All lanes:

NIH/3T3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Glutathione S Transferase mu antibody [EPR1936(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR1936(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This is an Anti-GSTM1 antibody, but it's immunogen shares 85% homology with GSTM5. Cross-reactivity with GSTM5 has not been confirmed experimentally.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

下記製品にもご興味をお持ちいただけるかもしれません:

AB277475

Human GSTM1 ELISA Kit

0

0 Reviews

製品ページを見る
こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

style
left-column

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Stable for 12 months at -20°C
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione S-transferase mu 1 also known as GSTM1 is an important enzyme involved in detoxification processes. This enzyme belongs to the glutathione S-transferases family which plays an important role in catalyzing the conjugation of glutathione to various electrophilic compounds. GSTM1 has a molecular mass of approximately 26 kDa and is primarily expressed in the liver but it is also present in other tissues such as the kidney and erythrocytes. GSTM1's active site involves a cysteine residue that facilitates the transfer of glutathione to potential harmful substances aiding in their subsequent excretion from the body.
Biological function summary

The enzyme contributes to maintaining cellular homeostasis by neutralizing toxins and protecting against oxidative stress. GSTM1 is part of a larger family of enzymes each with specific substrate affinities but all contributing to the detoxification of xenobiotics and carcinogens. This process is critically important to protect cells from environmental and oxidative damage. The enzyme highly expresses in liver tissue highlights its role in processing and eliminating harmful substances from the body.

Pathways

GSTM1 plays a significant role in the xenobiotic metabolism pathway where it participates in the detoxification and clearance of various xenobiotic substances. Another important pathway involving GSTM1 is the glutathione metabolism pathway. Within these pathways GSTM1 interacts with other glutathione S-transferases and proteins such as cytochrome P450s which also contribute to the metabolism of endogenous and exogenous compounds. The interaction with these proteins highlights its participation in a coordinated defense mechanism against chemical insults.

An absence or deficiency of GSTM1 is associated with an increased risk of conditions such as asthma and certain cancers particularly bladder cancer. The lack of GSTM1's detoxifying activity may increase susceptibility to environmental toxins and carcinogens. This enzyme together with other glutathione S-transferases plays a role in mitigating oxidative stress related to these conditions. Through its interactions with cytochrome P450 enzymes GSTM1 influences metabolic activation and deactivation of various potential carcinogens highlighting its relevance in disease prevention strategies.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276).
See full target information GSTM1
style
left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed36831305

2023

Role of C-Terminal Phosphorylation of Lamin A in DNA Damage and Cellular Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ao,Zhuping Wu,Zhiwei Liao,Juncong Lan,Jie Zhang,Pengfei Sun,Baohua Liu,Zimei Wang

Journal of cellular physiology 234:15885-15897 PubMed30741416

2019

Glutathione transferase P silencing promotes neuronal differentiation of retinal R28 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Sciarretta,Chiara Fulci,Camilla Palumbo,Katia Aquilano,Anna Pastore,Egidio Iorio,Daniele Lettieri-Barbato,Rosella Cicconi,Antonella Minutolo,Mariacristina Parravano,Marta Gilardi,Monica Varano,Anna Maria Caccuri

The Journal of neuroscience : the official journal 38:3466-3479 PubMed29507144

2018

Glutathione Conjugation at the Blood-CSF Barrier Efficiently Prevents Exposure of the Developing Brain Fluid Environment to Blood-Borne Reactive Electrophilic Substances.

Applications

Unspecified application

Species

Unspecified reactive species

Ingrid Kratzer,Nathalie Strazielle,Elodie Saudrais,Kati Mönkkönen,Céline Malleval,Sandrine Blondel,Jean-François Ghersi-Egea
View all publications
style
left-column
style
left-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
style
left-column
style
right-column

関連製品

Select an associated product type
Alternative Product
Primary Antibodies

AB268190

Anti-Glutathione S Transferase mu antibody [CPTC-GSTMu1-3] - BSA and Azide free

primary-antibodies

glutathione-s-transferase-mu-antibody-cptc-gstmu1-3-bsa-and-azide-free-ab268190

0

(0 reviews)

Alternative Product
Primary Antibodies

AB268069

Anti-Glutathione S Transferase mu antibody [CPTC-GSTMu1-3]

primary-antibodies

glutathione-s-transferase-mu-antibody-cptc-gstmu1-3-ab268069

0

(0 reviews)

Alternative Product
Primary Antibodies

AB53942

Anti-Glutathione S Transferase mu antibody

primary-antibodies

glutathione-s-transferase-mu-antibody-ab53942

4

(2 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com