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AB289369

Anti-Glutathione Peroxidase 2/GPX2 抗体 [HL125] - BSA and Azide free

Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free

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Rabbit Recombinant Monoclonal Glutathione Peroxidase 2/GPX2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human, Dog samples. Immunogen corresponding to Synthetic Peptide within Human Glutathione peroxidase 2.

別名を表示する

Glutathione peroxidase 2, GPx-2, GSHPx-2, Gastrointestinal glutathione peroxidase, Glutathione peroxidase-gastrointestinal, Glutathione peroxidase-related protein 2, Phospholipid hydroperoxide glutathione peroxidase GPX2, GPx-GI, GSHPx-GI, GPRP-2, GPX2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)

GPX2 antibody [HL125] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample : Paraffin-embedded mouse colon.
GPX2 stained by GPX2 antibody [HL125] (ab289369) diluted at 1 : 200.
Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)

Paraffin-embedded rat tissue stained for Glutathione Peroxidase 2/GPX2 protein with ab289369 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min .

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)

Paraffin-embedded dog kidney tissue stained for Glutathione Peroxidase 2/GPX2 protein at cytoplasm with ab289369 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

HL125

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Rat, Human, Mouse, Dog

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human Glutathione peroxidase 2. The exact immunogen used to generate this antibody is proprietary information.

P18283

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組合せ製品

Assay Kits

AB102530

Glutathione Peroxidase Assay Kit (Colorimetric)

Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (AB102530)

  • 5
  • 2
Primary Antibodies

AB22604

Anti-Glutathione Peroxidase 1 antibody

KO Validated

Western blot - Anti-Glutathione Peroxidase 1 antibody (AB22604)

  • 5
  • 23
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Primary Antibodies

AB183664

Anti-GPX8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPX8 antibody (AB183664)

  • 0
  • 0
Primary Antibodies

AB124954

Anti-TXNRD1 antibody [EPNCIR129]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "500-3000 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione Peroxidase 2 (GPX2) also known as GPX-GI or GPX2 weight belongs to the glutathione peroxidase family. This enzyme plays a mechanical role in reducing hydrogen peroxide by using glutathione which protects cells from oxidative damage. GPX2 is a selenoprotein with a molecular weight of approximately 22 kDa. It is expressed in the gastrointestinal tract particularly in the epithelial cells of the intestines and is also present in other tissues to a lesser extent.
Biological function summary

GPX2 functions as an antioxidant defense mechanism. It works in safeguarding cells by reducing reactive oxygen species and maintaining cellular redox balance. GPX2 is not part of a larger multiprotein complex but works closely with other antioxidant proteins to protect cells. Its expression helps regulate cellular homeostasis and supports tissue integrity especially under oxidative stress conditions.

Pathways

GPX2 continues its relevance in the glutathione metabolism pathway where it cooperates with other glutathione peroxidases and detoxifies peroxides. GPX2 also participates in the oxidative stress response pathway and interacts with proteins like GSSG and GPX1 enhancing the cellular antioxidant capacity. Its activity in these pathways underlines its significant role in maintaining cellular health and integrity by preventing oxidative damage.

GPX2 relates it to colorectal cancer and inflammatory bowel disease. In such conditions GPX2's expression levels are often altered indicating its role in cancer progression and inflammation management. The protein connects to other antioxidant enzymes such as GPX1 and SOD in the context of these diseases with changes in its function potentially impacting disease development and progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis (PubMed : 36608588, PubMed : 8428933). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed : 36608588, PubMed : 8428933). Cannot reduce phosphatidycholine hydroperoxide (PubMed : 8428933).
See full target information Glutathione peroxidase 2
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Abcam product promise

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