Anti-Glutathione 抗体 [D8]

• ICC/IF

AbReview30348****

Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (AB19534)

ab19534 staining Glutathione in cultured murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab19534 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/1000 dilution.

Image courtesy of Dr Mahesh Shivananjappa by Abreview.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (AB19534)

ab19534 staining Glutathione in human neutrophils by Immunocytochemistry/ Immunofluorescence.

Samples were fixed with 4% (w/v) paraformaldehyde in PBS, permeabilized with PBS containing 0.5% (w/v) saponin and 0.1% (w/v) bovine serum albumin, and then blocked with 1% (w/v) bovine serum albumin in PBS for 1 hour. ab19534 was used at 5μg/ml for 2 hours at room temperature. After washing with PBS, cells were incubated with Alexa 488-conjugated anti-mouse IgG at a 1/200 dilution.

Image from Lim SY et al, J Biol Chem. 2010 May 7;285(19):14377-88. Epub 2010 Mar 11, Fig 4.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Glutathione antibody [D8] (AB19534)

ab19534 staining glutathione in A549 cells treated with apocynin (ab120615), by ICC/IF. Increase in glutathione expression correlates with increased concentration of apocynin, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120615 (apocynin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19534 (10 μg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody.

• Flow Cyt

AbReview27288****

Flow Cytometry - Anti-Glutathione antibody [D8] (AB19534)

ab19534 at a 1/250 dilution detecting Glutathione in human monocytes by Flow Cytometry. An Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) secondary was used at a 1/500 dilution.

Image courtesy of Dr Mahesh Shivananjappa by Abreview.