JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB262716

Anti-Glutaminase C 抗体

Anti-Glutaminase C antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal Glutaminase antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLS.

別名を表示する

GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase

6 Images
Western blot - Anti-Glutaminase C antibody (AB262716)
  • WB

Supplier Data

Western blot - Anti-Glutaminase C antibody (AB262716)

This antibody specifically recognizes GAC but not the KGA isoform.

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Glutaminase C antibody (ab262716) at 1/5000 dilution

Lane 1:

Non-transfected MDA-MB-231 whole cell extracts at 30 µg

Lane 2:

GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts at 30 µg

Predicted band size: 73 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase C antibody (AB262716)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase C antibody (AB262716)

A549 cells stained for Glutaminase C using ab262716 at a 1/500 dilution (green) in ICC/IF.

Cells were fixed in 4% paraformaldehyde at RT for 15 mins.
Red : Histone H3K9ac (acetyl Lys9), a nucleus marker, stained by Histone H3K9ac (acetyl Lys9) antibody diluted at 1/500.
Blue : Hoechst 33342 staining.
Scale bar = 10 μm.

Immunoprecipitation - Anti-Glutaminase C antibody (AB262716)
  • IP

Supplier Data

Immunoprecipitation - Anti-Glutaminase C antibody (AB262716)

Immunoprecipitation of Glutaminase C protein from MDA-MB-231 whole cell extracts using 5 μg of ab262716.
Western blot analysis was performed using ab262716.
EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : Input

Lane 2 : Control IgG IP

Lane 3 : ab262716 IP

All lanes:

Immunoprecipitation - Anti-Glutaminase C antibody (ab262716)

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase C antibody (AB262716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase C antibody (AB262716)

Paraffin embedded mouse duodenum tissue stained for Glutaminase C using ab262716 at a 1/500 dilution in immunohistochemistry.

Antigen Retrieval : EDTA based, pH 8.0 buffer, 15mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase C antibody (AB262716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase C antibody (AB262716)

Paraffin embedded human colon carcinoma tissue stained for Glutaminase C using ab262716 at a 1/500 dilution in immunohistochemistry.

Antigen Retrieval : EDTA based, pH 8.0 buffer, 15mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase C antibody (AB262716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase C antibody (AB262716)

Paraffin embedded human breast carcinoma tissue stained for Glutaminase C using ab262716 at a 1/500 dilution in immunohistochemistry.

Antigen Retrieval : EDTA based, pH 8.0 buffer, 15mins.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

IHC-P, WB, ICC/IF, IP

applications

免疫原

Recombinant Fragment Protein within Human GLS. The exact immunogen used to generate this antibody is proprietary information.

O94925

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

下記製品にもご興味をお持ちいただけるかもしれません:

AB284572

Glutamine Synthetase Activity Assay Kit (Colorimetric)

0

0 Reviews

製品ページを見る
こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glutaminase C also known as kidney-type glutaminase or GLS2 is an enzyme with a high molecular mass of approximately 65 kDa. This enzyme belongs to the mitochondrial compartment and carries out the conversion of glutamine to glutamate releasing ammonia in the process. It is expressed in various tissues including brain kidney and liver where it plays an important role in cellular metabolism. The enzyme demonstrates increased expression levels under specific conditions where cellular proliferation and energy requirements are high.
Biological function summary

Glutaminase C acts as an important player in nitrogen metabolism and cellular bioenergetics. It functions within the mitochondria facilitating the glutaminolysis pathway which provides intermediates for the tricarboxylic acid (TCA) cycle. Although glutaminase C mainly acts as a monomer in certain conditions it might participate in multiprotein complexes that aid its regulation and activity. This regulatory mechanism plays a vital role in energy homeostasis and cellular growth demands.

Pathways

Glutaminase C is involved in the glutaminolysis and TCA cycle pathways contributing significantly to energy production and biosynthesis in proliferating cells. It works in concert with related proteins like glutamate dehydrogenase which further converts the produced glutamate into α-ketoglutarate feeding into the TCA cycle. These combined actions support cellular adaptations during increased metabolic demand especially in cancerous cells where metabolic shifts are noticeable.

Glutaminase C has a strong connection to cancer and neurological disorders. Cancer cells frequently exhibit heightened glutaminase C activity to sustain rapid growth associating with proteins such as c-Myc that drive metabolic reprogramming. Additionally in neurological ailments like epilepsy dysregulation of glutaminase C may alter neurotransmitter levels disturbing normal synaptic function. The enzyme's activity links to proteins involved in neuronal signaling suggesting its influence extends beyond mere metabolic processes.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed : 30239721, PubMed : 30575854, PubMed : 30970188).. Isoform 2. Lacks catalytic activity.
See full target information GLS
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 21:483-498 PubMed36185739

2022

HDAC I/IIb selective inhibitor Purinostat Mesylate combined with GLS1 inhibition effectively eliminates CML stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Qiu,Linyu Yang,Yunyu Feng,Zejiang Zhu,Ning Li,Li Zheng,Yuanyuan Sun,Cong Pan,Huandi Qiu,Xue Cui,Wei He,Fang Wang,Yuyao Yi,Minghai Tang,Zhuang Yang,Yunfan Yang,Zhihui Li,Lijuan Chen,Yiguo Hu

Cellular and molecular gastroenterology and hepatology 13:113-127 PubMed34530178

2021

Nrf2 Mutation/Activation Is Dispensable for the Development of Chemically Induced Mouse HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Mattu,Patrizia Zavattari,Marta Anna Kowalik,Marina Serra,Pia Sulas,Rajesh Pal,Elisabetta Puliga,Salvatore Sutti,Beatrice Foglia,Maurizio Parola,Emanuele Albano,Silvia Giordano,Andrea Perra,Amedeo Columbano
View all publications
style
left-column
style
left-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
style
left-column
style
right-column

関連製品

Select an associated product type
Alternative Product
Primary Antibodies

AB202027

Anti-Glutaminase C antibody [EPR19525]

primary-antibodies

glutaminase-c-antibody-epr19525-ab202027

0

(1 reviews)

Alternative Product
Primary Antibodies

AB223129

Anti-Glutaminase C antibody [EPR19525] - BSA and Azide free

primary-antibodies

glutaminase-c-antibody-epr19525-bsa-and-azide-free-ab223129

5

(2 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com