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リコンビナントRabMAb

Anti-Glutamate receptor 3/GluA3 抗体 [EP813Y] (ab40845)

Submit a review Q&A (3)References (21)
Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
  • Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y]
    Glutamate receptor 3/GluA3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP813Y] to Glutamate receptor 3/GluA3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Glutamate receptor 3/GluA3 aa 250-350. The exact sequence is proprietary.
    Database link: P42263

  • ポジティブ･コントロール

    • WB: Human brain tissue lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab40845の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).
特記事項
WB
1/1000 - 1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.

ターゲット情報

  • 機能

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

  • 関連疾患

    Defects in GRIA3 are the cause of mental retardation X-linked type 94 (MRX94) [MIM:300699]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRX94 patients have moderate mental retardation. Other variable features are macrocephaly, seizures, myoclonic jerks, autistic behavior, asthenic body habitus, distal muscle weakness and hyporeflexia.

  • 配列類似性

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA3 subfamily.

  • 翻訳後修飾

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-621 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-847 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.

  • 細胞内局在

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AMPA 3 antibody
    • AMPA selective glutamate receptor 3 antibody
    • AMPA-selective glutamate receptor 3 antibody
    • dJ1171F9.1 antibody
    • GluA3 antibody
    • GLUK3 antibody
    • GluR 3 antibody
    • GLUR C antibody
    • GLUR K3 antibody
    • GluR-3 antibody
    • GluR-C antibody
    • GluR-K3 antibody
    • GLUR3 antibody
    • GLURC antibody
    • Glutamate ionotropic receptor AMPA type subunit 3 antibody
    • Glutamate receptor 3 antibody
    • Glutamate receptor C antibody
    • Glutamate receptor ionotrophic AMPA 3 antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor subunit 3 antibody
    • Glutamate receptor, ionotropic, AMPA 3 antibody
    • GRIA 3 antibody
    • Gria3 antibody
    • GRIA3_HUMAN antibody
    • Ionotrophic Glutamate Receptor antibody
    • MRX94 antibody
    see all

画像

  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/1000 dilution (purified) + Human hippocampus lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 99 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    All lanes : Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/2000 dilution (purified)

    Lane 1 : Mouse hippocampus lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845) at 1/5000 dilution + human brain lysate at 10 µg

    Predicted band size: 99 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?

  • Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)
    Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (ab40845)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (21)

ab40845 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab40845 は 21 報の論文で使用されています。

  • Yokokawa T  et al. Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice. Physiol Rep 11:e15673 (2023). PubMed: 37078449
  • Shao S  et al. N-Cadherin Regulates GluA1-Mediated Depressive-Like Behavior in Adolescent Female Rat Offspring following Prenatal Stress. Neuroendocrinology 112:493-509 (2022). PubMed: 34348318
  • Giesen J  et al. Hippocampal AMPA- and NMDA-induced cGMP signals are mainly generated by NO-GC2 and are under tight control by PDEs 1 and 2. Eur J Neurosci 55:18-31 (2022). PubMed: 34902209
  • Hu MJ  et al. Acetylation of H3K27 activated lncRNA NEAT1 and promoted hepatic lipid accumulation in non-alcoholic fatty liver disease via regulating miR-212-5p/GRIA3. Mol Cell Biochem 477:191-203 (2022). PubMed: 34652536
  • Wright AL  et al. Riluzole does not ameliorate disease caused by cytoplasmic TDP-43 in a mouse model of amyotrophic lateral sclerosis. Eur J Neurosci 54:6237-6255 (2021). PubMed: 34390052
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

The datasheet is confusing because its difficult to determine if the antibody will specifically detect metaboropic or ionotropic glutamate receptor 3.

Read More
Answer

Thank you for your enquiry.

I have found that several years ago ab40845 was incorrectly listed as an antibody to metabotropic glutamate receptor 3. However it was then correct to list as ionotropic receptor. Unfortunately some indications from the old metabotropic datasheet survived, including those 2 citations and some alternative names.

So I can confirm ab40845 is specific for the ionotropic receptor. Also, the immunogen sequence is from amino acid 296 - 314 of the human protein.

I will contact our IT department to have the metabotropic related items removed from this datasheet to make things more clear.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

Question

Hi I would like to know if this antibody is selective for mGulR3 or if it cross reacts with mGluR2? Is it possible to have the peptide sequence so that I can check if this antibody is likely to cross react?

Read More
Answer

I'm afraid we are unable to provide the peptide sequence, however we have performed a sequence alignment of the immunogen with the mGluR2 protein and can confirm that ab40845 is not predicted to cross react with mGluR2 due to very low homology. Please do not hesitate to contact us if you require further information.

Read More

Question

Hello, I am wondering if you might run the epitope homology of this antibody to the X. tropicalis peptide sequence to see if this antibody might work in our system. I hope to use this antibody in a western blot application. Thanks. LFSKAVLLSSSDHNFVRKEIKFEGDLVLGGLFPINEKGGGMDECGRINEDRGIQRLEAML FAIDQINRDSSLLPGVKLGVHILDTCSRDTYALEQSLEFVRASLTKVDEAEYMCPDGSYA IQENSPLPIAGVIGGSYSSVSIQVANLLRLFHIPQISYASTSAKLSDKSRYDYFARTVPP DFYQAKAMAEILRFFNWTYVSTVASEGDYGETGIEAFEHEARLRNICIATSEKQTFFNKN NSAQYVLHLFCKNLISVIDKVGVGQNVSNNTDSIVKFDAYGDGIGLYNVFNFQYHNGKYS YLKIGQWAETLSLDVDIIQWSKSLVPTSQCSDPCAPNEMKNMQPGDVCCWICIPCEKYEY LADEFTCIDCGEGRWPTADLTSCFDLPEDYIKWEDAWAIGPVTIACLGFVCTCLVGGVFI KNNSTPLVKASGRELCYILLIGVFMCYCMTFFFIAKPSPVICTLRRLGLGTSFVVCYSAL LTKTNRIARIFSGVKDGAQRPKFISPKSQVVICLSLISVQIVVVSVWLMLEFPGTRRYTL PEKRETVILKCNVKDSSMLISLTYDVVLVILCTVYAFKTRKCPENFNEAKFIGFTMYTTC IIWLAFLPIFYVTSSDYRVQTTTMCISVSLSGFVVLGCLFAPKVHIILFQPQKN

Read More
Answer

Thank you for your enquiry. I did a sequence alignment between this antibody's immunogen to the sequence provided. Unfortunately, the alignment result showed poor amino acids sequence homology (less than 30% homology). I hope this information will be useful. Should you require any further information, please do not hesitate to contact me.

Read More

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