Anti-Glutamate Receptor 1 (AMPA subtype) 抗体 [N355/1] - N-terminal (ab174785)
![Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)](https://www.abcam.co.jp/ps/products/174/ab174785/Images/ab174785-316908-anti-glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)")
Key features and details
- Mouse monoclonal [N355/1] to Glutamate Receptor 1 (AMPA subtype) - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal
Glutamate Receptor 1 (AMPA subtype) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [N355/1] to Glutamate Receptor 1 (AMPA subtype) - N-terminal -
由来種
Mouse -
特異性
Does not cross- react with GluR2. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Cynomolgus monkey
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免疫原
Recombinant fragment within Rat Glutamate Receptor 1 (AMPA subtype) aa 1-400 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. within extracellular domain
Database link: P19490 -
ポジティブ･コントロール
- WB: Rat brain membrane lysate
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特記事項
The clone number has been updated from S355-1 to N355/1, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N355/1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab174785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB | (1) |
1/1000. Predicted molecular weight: 102 kDa.
ab174785 detects Glutamate Receptor 1 (AMPA subtype) in 20μg of Mouse brain membrane lysate using goat anti-mouse HRP conjugayed antibody as the secondary antibody. |
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
1/1000. Predicted molecular weight: 102 kDa. ab174785 detects Glutamate Receptor 1 (AMPA subtype) in 20μg of Mouse brain membrane lysate using goat anti-mouse HRP conjugayed antibody as the secondary antibody. |
ターゲット情報
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機能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
組織特異性
Widely expressed in brain. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
翻訳後修飾
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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参照データベース
- Entrez Gene: 2890 Human
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P19490 Rat
- Unigene: 519693 Human
- Unigene: 29971 Rat
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別名
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)
Immunocytochemistry/Immunofluorescence analysis using ab174785 at 1/100 in Neuroblastoma cell line (SK-N-BE). Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubation for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain 1/1000 for 60 min at RT; DAPI (blue) nuclear stain at 1/5000 for 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab174785 (D) Composite.
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)](https://www.abcam.co.jp/ps/products/174/ab174785/Images/ab174785-309951-anti-glutamate-receptor-1-ampa-subtype-antibody-n355-1-n-terminal-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785)Anti-Glutamate Receptor 1 (AMPA subtype) antibody [N355/1] - N-terminal (ab174785) at 1/2000 dilution + Rat Brain membrane lysate at 10 µg
Secondary
Goat Anti-Mouse HRP at 1/200 dilution
Predicted band size: 102 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab174785 は 7 報の論文で使用されています。
- Hu J et al. Constraint-induced movement therapy enhances AMPA receptor-dependent synaptic plasticity in the ipsilateral hemisphere following ischemic stroke. Neural Regen Res 16:319-324 (2021). PubMed: 32859791
- Hu J et al. Constraint-induced movement therapy improves functional recovery after ischemic stroke and its impacts on synaptic plasticity in sensorimotor cortex and hippocampus. Brain Res Bull 160:8-23 (2020). PubMed: 32298779
- Bingor A et al. Zeta Inhibitory Peptide attenuates learning and memory by inducing NO-mediated downregulation of AMPA receptors. Nat Commun 11:3688 (2020). PubMed: 32703948
- Notaras M et al. UPF2 leads to degradation of dendritically targeted mRNAs to regulate synaptic plasticity and cognitive function. Mol Psychiatry 25:3360-3379 (2020). PubMed: 31636381
- Kwon Y et al. ßPix heterozygous mice have defects in neuronal morphology and social interaction. Biochem Biophys Res Commun 516:1204-1210 (2019). PubMed: 31296385
- Szíber Z et al. Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover. Mol Biol Cell 28:285-295 (2017). WB ; Mouse . PubMed: 27852895
- Arora K et al. Behavioral alterations are associated with vitamin B12 deficiency in the transcobalamin receptor/CD320 KO mouse. PLoS One 12:e0177156 (2017). PubMed: 28545069