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リコンビナントRabMAb

Anti-Glutamate Receptor 1 (AMPA subtype) 抗体 [EPR19522] (ab183797)

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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
  • Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
  • Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
  • Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
  • Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19522] to Glutamate Receptor 1 (AMPA subtype)
  • Suitable for: WB, IHC-P, IP, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522]
    Glutamate Receptor 1 (AMPA subtype) 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19522] to Glutamate Receptor 1 (AMPA subtype)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IP, IHC-Frmore details
    適用なし: ICC

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse Glutamate Receptor 1 fragment recombinant protein; Mouse brain, hippocampus and cerebellum lysates; Rat brain, cerebellum and hippocampus lysates; Human cerebellum and fetal brain lysates. IHC-P: Mouse and rat hippocampus tissues. IHC-Fr: Mouse hippocampus tissue. IP: Mouse brain whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab183797の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC is recommended for mouse and rat only.
IP
1/30.
IHC-Fr
1/100.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20)

IHC is recommended for mouse and rat only
特記事項
WB
1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC is recommended for mouse and rat only.
IP
1/30.
IHC-Fr
1/100.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20)

IHC is recommended for mouse and rat only
追加情報
Is unsuitable for ICC.

ターゲット情報

  • 機能

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

  • 組織特異性

    Widely expressed in brain.

  • 配列類似性

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.

  • 翻訳後修飾

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.

  • 細胞内局在

    Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • GLUR 1 antibody
    • GLUR A antibody
    • AMPA 1 antibody
    • AMPA selective glutamate receptor 1 antibody
    • AMPA-selective glutamate receptor 1 antibody
    • GluA1 antibody
    • GLUH1 antibody
    • GluR K1 antibody
    • GluR-1 antibody
    • GluR-A antibody
    • GluR-K1 antibody
    • GLUR1 antibody
    • GLURA antibody
    • GluRK1 antibody
    • Glutamate receptor 1 antibody
    • Glutamate receptor ionotropic AMPA 1 antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor, ionotropic, AMPA 1 antibody
    • Gria1 antibody
    • GRIA1_HUMAN antibody
    • HBGR1 antibody
    • MGC133252 antibody
    • OTTHUMP00000160643 antibody
    • OTTHUMP00000165781 antibody
    • OTTHUMP00000224241 antibody
    • OTTHUMP00000224242 antibody
    • OTTHUMP00000224243 antibody
    see all

画像

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/5000 dilution

    Lane 1 : Mouse Glutamate Receptor 1 fragment recombinant protein
    Lane 2 : Mouse Glutamate Receptor 2 fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 102 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 2 seconds; Lane 2: 3 minutes.

    Mouse Glutamate Receptor 1 fragment recombinant protein contains aa19-184 with a GST/His-Tag®. Mouse Glutamate Receptor 2 fragment recombinant protein contains aa25-288 with a His-Tag®.

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat liver lysate
    Lane 3 : Mouse hippocampus lysate
    Lane 4 : Mouse cerebellum lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat cerebellum lysate
    Lane 7 : Rat hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 102 kDa
    Observed band size: 102 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1 and 2: 30 seconds; Lane 3, 4, 5, 6 and 7: 15 seconds.

    Rodent Glutamate Receptor 1 is widely expressed in brain and represents the predominant excitatory neurotransmitter system but not in liver.

    Negative control: Rat liver (PMID: 2480522).

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human muscle lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 102 kDa
    Observed band size: 102 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human Glutamate Receptor 1 is widely expressed in brain and represents the predominant excitatory neurotransmitter system but not in muscle.

    Negative control: human muscle (PMID: 1652753).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse hippocampus was observed [PMID: 15723058]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on mouse liver is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)

    Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat hippocampus is observed [PMID: 15723058]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on rat stomach is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on mouse hippocampus was observed.  The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on mouse hippocampus was observed.  The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077  at 1/1000 dilution.

  • Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)

    Glutamate Receptor 1 (AMPA subtype) was immunoprecipitated from 0.35 mg of Mouse brain whole cell lysate with ab183797 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183797 at 1/1000 dilution. VeriBlot for IP Detection Reaction (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse brain whole cell lysate, 10ug (Input).

    Lane 2: ab183797 IP in Mouse brain whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183797 in Mouse brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)
    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)

参考文献 (24)

ab183797 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab183797 は 24 報の論文で使用されています。

  • Zhang Y  et al. Testosterone reduces hippocampal synaptic damage in an androgen receptor-independent manner. J Endocrinol 260:N/A (2024). PubMed: 37991884
  • Yao XP  et al. Adaptor protein MyD88 confers the susceptibility to stress via amplifying immune danger signals. Brain Behav Immun 108:204-220 (2023). PubMed: 36496170
  • Qin T  et al. Combined effects of EMP and RF field on emotional behavior in mice. Front Public Health 11:1087161 (2023). PubMed: 37006533
  • Fu Y  et al. Hippocampal BAIAP2 prevents chronic mild stress-induced depression-like behaviors in mice. Front Psychiatry 14:1192379 (2023). PubMed: 37234209
  • Qi F  et al. VEGF-A in serum protects against memory impairment in APP/PS1 transgenic mice by blocking neutrophil infiltration. Mol Psychiatry 28:4374-4389 (2023). PubMed: 37280283
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

Western blot abreview for Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Zebra finch Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Saina Gao

Verified customer

投稿 Jan 06 2024

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