Anti-Glutamate Receptor 1 (AMPA subtype) 抗体 [EPR19522]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse hippocampus was observed [PMID : 15723058]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on mouse hippocampus was observed. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat hippocampus is observed [PMID : 15723058]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on mouse hippocampus was observed. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab150077 at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on rat stomach is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on mouse liver is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Glutamate Receptor 1 (AMPA subtype) was immunoprecipitated from 0.35 mg of Mouse brain whole cell lysate with ab183797 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183797 at 1/1000 dilution. VeriBlot for IP Detection Reaction (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse brain whole cell lysate, 10ug (Input).

Lane 2 : ab183797 IP in Mouse brain whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183797 in Mouse brain whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797)

Predicted band size: 102 kDa

false

• WB

Lab

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver (PMID : 2480522).human muscle (PMID : 1652753).

Bands around 300kDa in lane 4, lane 6 and lane 8 may due to protein aggregation.

All lanes:

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

human skeletal muscle tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Lane 7:

Rat cerebellum tissue at 20 µg

Lane 8:

Rat hippocampus tissue lysate at 20 µg

Lane 9:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 101 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human Glutamate Receptor 1 is widely expressed in brain and represents the predominant excitatory neurotransmitter system but not in muscle.

Negative control : human muscle (PMID : 1652753).

All lanes:

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 20 µg

Lane 2:

Human muscle lysate at 20 µg

Lane 3:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 102 kDa

Observed band size: 102 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 2 seconds; Lane 2 : 3 minutes.

Mouse Glutamate Receptor 1 fragment recombinant protein contains aa19-184 with a GST/His-Tag^®. Mouse Glutamate Receptor 2 fragment recombinant protein contains aa25-288 with a His-Tag^®.

All lanes:

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/5000 dilution

Lane 1:

Mouse Glutamate Receptor 1 fragment recombinant protein at 0.01 µg

Lane 2:

Mouse Glutamate Receptor 2 fragment recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 102 kDa

false

• WB

Lab

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with Mouse GRIA2, GRIA3 and GRIA4.

All lanes:

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution

Lane 1:

293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with Mouse full length GRIA1 expression vector containing a c-term myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with Mouse full length GRIA2 expression vector containing a c-term myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with Mouse full length GRIA3 expression vector containing a c-term myc-His-tag®, whole cell lysate at 20 µg

Lane 5:

293T transfected with Mouse full length GRIA4 expression vector containing a c-term myc-His-tag®, whole cell lysate at 20 µg

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 1 - 5:

Goat anti-mouse IgG (H+L), Peroxidase conjugated at 1/10000 dilution

Observed band size: 101 kDa

false

Exposure time: 20s

• WB

Supplier Data

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (AB183797)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 and 2 : 30 seconds; Lane 3, 4, 5, 6 and 7 : 15 seconds.

Rodent Glutamate Receptor 1 is widely expressed in brain and represents the predominant excitatory neurotransmitter system but not in liver.

Negative control : Rat liver (PMID : 2480522).

All lanes:

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat liver lysate at 20 µg

Lane 3:

Mouse hippocampus lysate at 20 µg

Lane 4:

Mouse cerebellum lysate at 20 µg

Lane 5:

Rat brain lysate at 20 µg

Lane 6:

Rat cerebellum lysate at 20 µg

Lane 7:

Rat hippocampus lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 102 kDa

Observed band size: 102 kDa

false