Anti-Glutamate Receptor 1 (AMPA subtype) 抗体 [E308] (ab32436)
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)](https://www.abcam.co.jp/ps/products/32/ab32436/Images/ab32436-44844-anti-glutamate-receptor-1-ampa-subtype-antibody-e308-western-blot.jpg "Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E308] to Glutamate Receptor 1 (AMPA subtype)
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308]
Glutamate Receptor 1 (AMPA subtype) 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E308] to Glutamate Receptor 1 (AMPA subtype) -
由来種
Rabbit -
特異性
This antibody is specific for Glutamate Receptor 1 (AMPA subtype). -
アプリケーション
適用あり: WB, IHC-P, IPmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Glutamate Receptor 1 (AMPA subtype) aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- WB: SH-SY5Y cell lysate. IHC: human brain tissue. IP: SH-SY5Y cell lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E308 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32436の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 101 kDa).
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| IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/50.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 101 kDa). |
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IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
1/50. |
追加情報
Is unsuitable for Flow Cyt or ICC/IF.
ターゲット情報
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機能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
組織特異性
Widely expressed in brain. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
翻訳後修飾
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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参照データベース
- Entrez Gene: 2890 Human
- Omim: 138248 Human
- SwissProt: P42261 Human
- Unigene: 519693 Human
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別名
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
画像
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436) at 1/1000 dilution + SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Predicted band size: 101 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)](https://www.abcam.co.jp/ps/products/32/ab32436/Images/ab32436-5-anti-glutamate-receptor-1-ampa-subtype-antibody-e308-immunoprecipitation-shsy5y-human.jpg)
Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)Purified ab32436 at 1/50 dilution (2µg) immunoprecipitating Glutamate Receptor 1 (AMPA subtype) in SH-SY5Y whole cell lysate.
Lane 1 (input): SY-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab32436 + SH-SY5Y whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32436 in SH-SY5Y whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 106 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)](https://www.abcam.co.jp/ps/products/32/ab32436/Images/ab32436-44843-anti-glutamate-receptor-1-ampa-subtype-antibody-e308-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)Ab32436, at a 1/50 dilution, staining Glutamate Receptor 1 (AMPA subtype) in paraffin embedded human brain tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)](https://www.abcam.co.jp/ps/products/32/ab32436/Images/ab32436-44842-anti-glutamate-receptor-1-ampa-subtype-antibody-e308-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)Image from van Vuurden DG et al., PLoS One. 2009 Jun 18;4(6):e5953. Fig 2.; doi:10.1371/journal.pone.0005953; June 18, 2009, PLoS ONE 4(6): e5953.Immunohistochemical analysis of Human hippocampus tissue, staining Glutamate Receptor 1 (AMPA subtype) with ab32436.
Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Samples were blocked with 10% normal goat serum for 30 minutes before incubating with primary antibody (1/20) for 2 hours. A biotinylated anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB. -
![Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)](https://www.abcam.co.jp/ps/products/32/ab32436/Images/ab32436-4-benefits-of-recombinant-antibodies.png)
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab32436 は 7 報の論文で使用されています。
- Zhao DP et al. Sagacious confucius' pillow elixir ameliorates Dgalactose induced cognitive injury in mice via estrogenic effects and synaptic plasticity. Front Pharmacol 13:971385 (2022). PubMed: 36249769
- Gregory JM et al. Dysregulation of AMPA receptor subunit expression in sporadic ALS post-mortem brain. J Pathol 250:67-78 (2020). PubMed: 31579943
- Taylor BK et al. Opioid receptors inhibit the spinal AMPA receptor Ca2+ permeability that mediates latent pain sensitization. Exp Neurol 314:58-66 (2019). PubMed: 30660616
- Emanuele M et al. Exogenous Alpha-Synuclein Alters Pre- and Post-Synaptic Activity by Fragmenting Lipid Rafts. EBioMedicine 7:191-204 (2016). PubMed: 27322472
- Qiu S et al. GluA1 phosphorylation contributes to postsynaptic amplification of neuropathic pain in the insular cortex. J Neurosci 34:13505-15 (2014). PubMed: 25274827
- Plaza-Zabala A et al. An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions and after cocaine exposure. Neurosci Lett 557 Pt B:101-6 (2013). WB ; Rat . PubMed: 24262606
- van Vuurden DG et al. Attenuated AMPA receptor expression allows glioblastoma cell survival in glutamate-rich environment. PLoS One 4:e5953 (2009). IHC-P ; Human . PubMed: 19536293