Anti-Glutamate Receptor 1 (AMPA subtype) 抗体 (ab116616)
Key features and details
- Goat polyclonal to Glutamate Receptor 1 (AMPA subtype)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Glutamate Receptor 1 (AMPA subtype) antibody
Glutamate Receptor 1 (AMPA subtype) 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Glutamate Receptor 1 (AMPA subtype) -
由来種
Goat -
特異性
ab116616 is expected to recognize both reported isoforms (NP_000818.2; NP_001107655.1). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Dog
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免疫原
Synthetic peptide:
KIMQQWKNSDARD
with a Cysteine residue linker, corresponding to internal sequence amino acids 265-277 of Human Glutamate Receptor 1 (AMPA subtype) (NP_000818.2; NP_001107655.1). -
ポジティブ･コントロール
- Human, Mouse and Rat brain cerebellum lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab116616 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab116616の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
組織特異性
Widely expressed in brain. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
翻訳後修飾
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
細胞内局在
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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参照データベース
- Entrez Gene: 2890 Human
- Omim: 138248 Human
- SwissProt: P42261 Human
- Unigene: 519693 Human
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別名
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
画像
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab116616)Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab116616) at 0.1 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab116616 は論文での使用が確認できていません。