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リコンビナントRabMAb

Anti-GluN3A+GluN3B 抗体 [EPR25287-45] (BSA and Azide free) (ab302535)

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Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)
  • Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
  • Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
  • Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25287-45] to NR3A + NR3B - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free)

  • 製品の詳細

    Rabbit monoclonal [EPR25287-45] to NR3A + NR3B - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: ICC/IF or IHC-Fr

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Tissue lysates: Rat and mouse thalamus, rat P0 and mouse P1 brain. Whole cell lysates: HEK-293T cells transfected with a rat GluN3A expression vector containing a his tag, HEK-293T cells transfected with a rat GluN3B expression vector containing a his tag. IHC-P: Mouse and rat cerebrum tissue.

  • 特記事項

    ab302535 is a carrier free version of AB302534. 

    ab302534 does not react in: WBand IHC-P with human tissues; IHC-Fr and ICC with mouse and rat species. 

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302535の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 127 kDa).
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 127 kDa).
追加情報
Is unsuitable for ICC/IF or IHC-Fr.

ターゲット情報

  • 細胞内局在

    NR3A: Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membrane. NR3B: Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Requires the presence of GRIN1 to be targeted at the plasma membrane.

  • 参照データベース

    see all

  • 製品の状態

    NR3B: A subunit of the N-methyl-D-aspartate (NMDA) receptor, which belongs to the superfamily of glutamate-regulated ion channels that are present in neurons throughout the central nervous system.

画像

  • Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)
    Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (ab302535)
    All lanes : Anti-GluN3A+GluN3B antibody [EPR25287-45] (ab302534) at 1/1000 dilution

    Lane 1 : Rat thalamus tissue lysate
    Lane 2 : Rat P0 brain tissue lysate
    Lane 3 : Rat heart tissue lysate
    Lane 4 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 127 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab302534, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    Negative control: heart, spleen (PMID: 7472412).

    Samples are non-boiled as boiling may cause protein aggregates.

  • Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
    Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
    All lanes : Anti-GluN3A+GluN3B antibody [EPR25287-45] (ab302534) at 1000 cells

    Lane 1 : Mouse thalamus tissue lysate
    Lane 2 : Mouse P1 brain tissue lysate
    Lane 3 : Mouse heart tissue lysate
    Lane 4 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 127 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab302534, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

    Negative controls: heart, spleen (PMID: 7472412). 

    Samples are non-boiled as boiling may cause protein aggregates

  • Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
    Western blot - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
    All lanes : Anti-GluN3A+GluN3B antibody [EPR25287-45] (ab302534) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a his tag, whole cell lysate,
    Lane 2 : HEK-293T cells transfected with a rat GluN3A expression vector containing a his tag, whole cell lysate
    Lane 3 : HEK-293T cells transfected with a rat GluN3B expression vector containing a his tag, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 127 kDa
    Observed band size: 130, 125 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab302534, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

    This antibody reacts with rat GluN3A and GluN3B.

    Samples are non-boiled as boiling may cause protein aggregates.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)

    This data was developed using ab302534, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling NR3A and NR3B with AB302534 at 1/200 dolition (2.75 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Postive staining on mouse cerebrum (PMID: 7472412). The section was incubated with AB302534 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)

    This data was developed using ab302534, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling NR3A and NR3B with AB302534 at 1/200 dolition (2.75 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Postive staining on rat cerebrum (PMID: 7472412). The section was incubated with AB302534 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)

    This data was developed using ab302534, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling NR3A and NR3B with AB302534 at 1/200 dolition (2.75 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse cardiac muscle. The section was incubated with AB302534 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)

    This data was developed using ab302534, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling NR3A and NR3B with AB302534 at 1/200 dolition (2.75 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat cardiac muscle. The section was incubated with AB302534 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

  • Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)
    Anti-GluN3A+GluN3B antibody [EPR25287-45] (BSA and Azide free) (AB302535)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab302535 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302535 は論文での使用が確認できていません。

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