Anti-GluK5 抗体 (ab67408)
Key features and details
- Rabbit polyclonal to GluK5
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GluK5 antibody
GluK5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GluK5 -
由来種
Rabbit -
特異性
ab67408 does not cross-react with GRIK4. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human GluK5 (C terminal).
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ポジティブ･コントロール
- Human brain tissue and brain tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67408の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 109 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 109 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > domoate > L-glutamate >> AMPA >> NMDA = 1S,3R-ACPD. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK5 subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2901 Human
- Omim: 600283 Human
- SwissProt: Q16478 Human
- Unigene: 367799 Human
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別名
- EAA 2 antibody
- EAA2 antibody
- Excitatory amino acid receptor 2 antibody
see all
画像
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Western blot - Anti-GluK5 antibody (ab67408)Lane 1 : Anti-GluK5 antibody (ab67408) at 0.5 µg/ml
Lane 2 : Anti-GluK5 antibody (ab67408) at 1 µg/ml
Lane 3 : Anti-GluK5 antibody (ab67408) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 109 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluK5 antibody (ab67408)ab67408 at 2.5µg/ml staining of GluK5 in human brain tissue by IHC-P.
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Immunocytochemistry/ Immunofluorescence - Anti-GluK5 antibody (ab67408)Immunofluorescence of GluK5 in Human Brain cells using ab67408 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab67408 は 2 報の論文で使用されています。
- Zhu Z et al. Dynamics of the Transcriptome during Human Spermatogenesis: Predicting the Potential Key Genes Regulating Male Gametes Generation. Sci Rep 6:19069 (2016). PubMed: 26753906
- Funk JA et al. Characterization of Renal Toxicity in Mice Administered the Marine Biotoxin Domoic Acid. J Am Soc Nephrol N/A:N/A (2014). PubMed: 24511141