Anti-GLUD1 + GLUD2 抗体 [EPR11370] (ab168352)
![Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)](https://www.abcam.co.jp/ps/products/168/ab168352/Images/ab168352-166622-anti-glud1-antibody-epr11370-western-blot.jpg "Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11370] to GLUD1 + GLUD2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GLUD1 + GLUD2 antibody [EPR11370]
GLUD1+GLUD2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11370] to GLUD1 + GLUD2 -
由来種
Rabbit -
特異性
Ab168352 immunogen shows 93% identity to human GLUD2.
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アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human fetal liver tissue lysate and HepG2 whole cell lysate (ab7900). IHC-P: Human heart and liver tissues. ICC/IF: HepG2 cells. Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11370 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168352の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/2000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| 特記事項 |
|---|
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Flow Cyt (Intra)
1/2000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/50 - 1/100. |
ターゲット情報
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細胞内局在
GLUD1: Mitochondrion matrix. GLUD2: Mitochondrion matrix. -
参照データベース
- Entrez Gene: 2746 Human
- Entrez Gene: 2747 Human
- Entrez Gene: 104277 Mouse
- Entrez Gene: 14661 Mouse
- Entrez Gene: 24399 Rat
- GenBank: NM_012084.3 Human
- Omim: 138130 Human
- Omim: 300144 Human
see all
画像
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Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)All lanes : Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)](https://www.abcam.co.jp/ps/products/168/ab168352/Images/ab168352-168970-anti-glud1-antibody-epr11370-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)ab168352 showing +ve staining in Human normal pancreas.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)](https://www.abcam.co.jp/ps/products/168/ab168352/Images/ab168352-166621-anti-glud1-antibody-epr11370-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)Immunofluorescent analysis of HepG2 cells labeling Glutamate Dehydrogenase with ab168352 at 1/50 dilution. -
![Flow Cytometry (Intracellular) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)](https://www.abcam.co.jp/ps/products/168/ab168352/Images/ab168352-280399-anti-glud1-antibody-epr11370-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GLUD1 (red) with ab168352 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)](https://www.abcam.co.jp/ps/products/168/ab168352/Images/ab168352-168971-anti-glud1-antibody-epr11370-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)ab168352 showing +ve staining in Human normal uterus.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)](https://www.abcam.co.jp/ps/products/168/ab168352/Images/ab168352-168969-anti-glud1-antibody-epr11370-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)ab168352 showing +ve staining in Human normal kidney.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)](https://www.abcam.co.jp/ps/products/168/ab168352/Images/ab168352-166620-anti-glud1-antibody-epr11370-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)](https://www.abcam.co.jp/ps/products/168/ab168352/Images/ab168352-166619-anti-glud1-antibody-epr11370-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)](https://www.abcam.co.jp/ps/products/168/ab168352/Images/ab168352-9-benefits-of-recombinant-antibodies.png)
Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab168352 は 9 報の論文で使用されています。
- Pan L et al. TBK1 participates in glutaminolysis by mediating the phosphorylation of RIPK3 to promote endotoxin tolerance. Mol Immunol 147:101-114 (2022). PubMed: 35533409
- Zhang Z et al. Identification and validation of an immune signature associated with EMT and metabolic reprogramming for predicting prognosis and drug response in bladder cancer. Front Immunol 13:954616 (2022). PubMed: 35958586
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
- Sharpley MS et al. Metabolic plasticity drives development during mammalian embryogenesis. Dev Cell 56:2329-2347.e6 (2021). PubMed: 34428399
- He J et al. Methylcrotonoyl-CoA Carboxylase 2 Promotes Proliferation, Migration and Invasion and Inhibits Apoptosis of Prostate Cancer Cells Through Regulating GLUD1-P38 MAPK Signaling Pathway. Onco Targets Ther 13:7317-7327 (2020). PubMed: 32801758
- Sun N et al. Glutamine affects T24 bladder cancer cell proliferation by activating STAT3 through ROS and glutaminolysis. Int J Mol Med 44:2189-2200 (2019). PubMed: 31661119
- Craze ML et al. Glutamate dehydrogenase (GLUD1) expression in breast cancer. Breast Cancer Res Treat N/A:N/A (2018). PubMed: 30470977
- Li W et al. HIF-2a regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma. J Cell Mol Med 21:2896-2908 (2017). WB . PubMed: 28544376
- Vázquez-Martínez O et al. Restricted feeding modulates the daily variations of liver glutamate dehydrogenase activity, expression, and histological location. Exp Biol Med (Maywood) 242:945-952 (2017). PubMed: 28440738