Anti-Glucose Transporter GLUT4 抗体 [mAbcam48547] (ab48547)
![Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)](https://www.abcam.co.jp/ps/products/48/ab48547/Images/ab48547-61041-anti-glucose-transporter-glut4-antibody-mabcam48547-western-blot.jpg "Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)")
Key features and details
- Mouse monoclonal [mAbcam48547] to Glucose Transporter GLUT4
- Suitable for: WB
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG2b
製品の概要
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製品名
Anti-Glucose Transporter GLUT4 antibody [mAbcam48547]
Glucose Transporter GLUT4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [mAbcam48547] to Glucose Transporter GLUT4 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra) -
種交差性
交差種: Mouse, Recombinant fragment
交差が予測される動物種: Rat, Sheep, Rabbit, Goat, Horse, Cow
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免疫原
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ポジティブ･コントロール
- WB: ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
mAbcam48547 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48547の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 54 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 54 kDa). |
追加情報
Is unsuitable for Flow Cyt (Intra).
ターゲット情報
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機能
Insulin-regulated facilitative glucose transporter. -
組織特異性
Skeletal and cardiac muscles; brown and white fat. -
関連疾患
Diabetes mellitus, non-insulin-dependent -
配列類似性
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
翻訳後修飾
Sumoylated. -
細胞内局在
Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. - Information by UniProt
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参照データベース
- Entrez Gene: 282359 Cow
- Entrez Gene: 20528 Mouse
- Entrez Gene: 25139 Rat
- SwissProt: Q27994 Cow
- SwissProt: P14142 Mouse
- SwissProt: P19357 Rat
- Unigene: 10661 Mouse
- Unigene: 1314 Rat
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別名
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
画像
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Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547) at 5 µg/ml + Partial tagged recombinant protein to GLUT4 at 0.1 µg
Secondary
Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab48547 は 9 報の論文で使用されています。
- Silva AFR et al. The Impact of Resveratrol-Enriched Bread on Cardiac Remodeling in a Preclinical Model of Diabetes. Antioxidants (Basel) 12:N/A (2023). PubMed: 37237932
- Rodríguez-Castelán J et al. Preventive Effect of Molecular Iodine in Pancreatic Disorders from Hypothyroid Rabbits. Int J Mol Sci 24:N/A (2023). PubMed: 37834351
- Panahi M et al. Insulin-Independent and Dependent Glucose Transporters in Brain Mural Cells in CADASIL. Front Genet 11:1022 (2020). PubMed: 33101365
- Ono E et al. Sweat glucose and GLUT2 expression in atopic dermatitis: Implication for clinical manifestation and treatment. PLoS One 13:e0195960 (2018). PubMed: 29677207
- Li J et al. Inhibition of insulin/PI3K/AKT signaling decreases adipose Sortilin 1 in mice and 3T3-L1 adipocytes. Biochim Biophys Acta Mol Basis Dis 1863:2924-2933 (2017). PubMed: 28844948
- Joannides CN et al. Dapagliflozin improves insulin resistance and glucose intolerance in a novel transgenic rat model of chronic glucose overproduction and glucose toxicity. Diabetes Obes Metab 19:1135-1146 (2017). PubMed: 28244693
- Mano Y et al. Correlation between biological marker expression and fluorine-18 fluorodeoxyglucose uptake in hepatocellular carcinoma. Am J Clin Pathol 142:391-7 (2014). Human . PubMed: 25125631
- Karim S et al. Dysregulated hepatic expression of glucose transporters in chronic disease: contribution of semicarbazide-sensitive amine oxidase to hepatic glucose uptake. Am J Physiol Gastrointest Liver Physiol 307:G1180-90 (2014). PubMed: 25342050
- Mattmiller SA et al. Glucose transporter and hypoxia-associated gene expression in the mammary gland of transition dairy cattle. J Dairy Sci 94:2912-22 (2011). PubMed: 21605761