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Anti-Glucose Transporter GLUT2 抗体 [5D1] (ab85715)

Reviews (4)Q&A (3)References (9)
Western blot - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)
  • Flow Cytometry - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)

Key features and details

  • Mouse monoclonal [5D1] to Glucose Transporter GLUT2
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Glucose Transporter GLUT2 antibody [5D1]
    Glucose Transporter GLUT2 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [5D1] to Glucose Transporter GLUT2

  • 由来種

    Mouse

  • 特異性

    ab85715 recognizes Glucose Transporter GLUT2 expression on hGlut2-transfected NS0 cells but not on control transfectants. Detects Glut2 on the surface of the Human intestinal cell line Caco2 in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with hGlut1 or hGlut3.

  • アプリケーション

    適用あり: IHC-P, Flow Cyt, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human Glucose Transporter GLUT2. Recombinant protein corresponding to Met1-Val524 from human GLUT 2 expressed in NS0 cells.

  • ポジティブ･コントロール

    • Liver tissue. Flow Cytometry: HepG2 cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab85715の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
Use a concentration of 10 µg/ml.
Flow Cyt
Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
特記事項
IHC-P
Use a concentration of 10 µg/ml.
Flow Cyt
Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

ターゲット情報

  • 機能

    Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.

  • 組織特異性

    Liver, insulin-producing beta cell, small intestine and kidney.

  • 関連疾患

    Fanconi-Bickel syndrome

  • 配列類似性

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

  • 翻訳後修飾

    N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • liver antibody
      • Glucose Transporter 2 antibody
      • Glucose Transporter GLUT2 antibody
      • Glucose transporter type 2 antibody
      • Glucose transporter type 2 liver antibody
      • Glucose transporter, liver/islet antibody
      • GLUT-2 antibody
      • GLUT2 antibody
      • GTR2_HUMAN antibody
      • GTT2 antibody
      • SLC2A2 antibody
      • Solute carrier family 2 (facilitated glucose transporter) member 2 antibody
      • Solute carrier family 2 facilitated glucose transporter member 2 antibody
      • Solute carrier family 2, facilitated glucose transporter member 2 antibody
      see all

    画像

    • Western blot - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)
      Western blot - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)
      Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 57 kDa
      Observed band size: 57 kDa


      Exposure time: 8 minutes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)
      ab85715, at 10 µg/ml, staining Glucose Transporter GLUT2 in formalin-fixed, paraffin-embedded human Liver tissue by Immunohistochemistry.
    • Flow Cytometry - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)
      Flow Cytometry - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)
      Overlay histogram showing HepG2 cells stained with ab85715 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85715, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    参考文献 (9)

    ab85715 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab85715 は 9 報の論文で使用されています。

    • Pittala S  et al. The VDAC1-based R-Tf-D-LP4 Peptide as a Potential Treatment for Diabetes Mellitus. Cells 9:N/A (2020). PubMed: 32093016
    • Rogers RC  et al. Evidence that hindbrain astrocytes in the rat detect low glucose with a glucose transporter 2-phospholipase C-calcium release mechanism. Am J Physiol Regul Integr Comp Physiol 318:R38-R48 (2020). PubMed: 31596114
    • Villalva-Pérez JM  et al. Characterization of Huh7 cells after the induction of insulin resistance and post-treatment with metformin. Cytotechnology 72:499-511 (2020). PubMed: 32409919
    • Fujino M  et al. Expression of glucose transporter-1 is correlated with hypoxia-inducible factor 1a and malignant potential in pancreatic neuroendocrine tumors. Oncol Lett 12:3337-3343 (2016). IHC-P ; Human . PubMed: 27900001
    • Nguyen NQ  et al. Upregulation of intestinal glucose transporters after Roux-en-Y gastric bypass to prevent carbohydrate malabsorption. Obesity (Silver Spring) 22:2164-71 (2014). PubMed: 24990218
    • Mano Y  et al. Correlation between biological marker expression and fluorine-18 fluorodeoxyglucose uptake in hepatocellular carcinoma. Am J Clin Pathol 142:391-7 (2014). Human . PubMed: 25125631
    • Kern M  et al. Liver-restricted Repin1 deficiency improves whole-body insulin sensitivity, alters lipid metabolism, and causes secondary changes in adipose tissue in mice. Diabetes 63:3295-309 (2014). PubMed: 24760138
    • Deane AM  et al. The effects of critical illness on intestinal glucose sensing, transporters, and absorption. Crit Care Med 42:57-65 (2014). PubMed: 23963126
    • Vargas N  et al. TAT-Mediated Transduction of MafA Protein In Utero Results in Enhanced Pancreatic Insulin Expression and Changes in Islet Morphology. PLoS One 6:e22364 (2011). IHC-FoFr ; Mouse . PubMed: 21857924

    レビューと Q&A

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT2 antibody [5D1]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (B16 Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    Yes - 0.1% Triton-X100 in PBS 10'
    Specification
    B16 Pancreas
    Blocking step
    Bloxall as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 05 2022

    Western blot abreview for Anti-Glucose Transporter GLUT2 antibody [5D1]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (B16 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    B16 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 03 2019

    Western blot abreview for Anti-Glucose Transporter GLUT2 antibody [5D1]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Panc1)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycine Gel, PVDF membrane)
    Loading amount
    40 µg
    Specification
    Panc1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 03 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose Transporter GLUT2 antibody [5D1]

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Pancreas)
    Specification
    Pancreas
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - PBST (0.02% Tween)
    Blocking step
    (agent) for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Tirosh Shapira

    Verified customer

    投稿 Mar 16 2010

    Question

    Thank you for providing me the information on the pancreas tissue lysate ab29816 (lot # GR26918-2). Based on our conversation by phone in order to try to determine the cause of the problem, I agreed to run the pancreas tissue lysate in a gel and stained it with Coomassie blue before and after the transfer. The results are attached. Since I did have very limited materialleft, I was only able to add 10 ug of lysate per lane. I also added the liver tissue lysate (Abcam ab29889 lot GR67555-1) that was managed under the same conditions than the pancreas tissue.

    After the staining with Coomassie blue, the results showed that I can clearly see the material in the liver sample, but I was unable to see it in the pancreas. The amount of material in the pancreas sample is clearly significantly less than the liver sample and than the 3.75 ug/uL that is on the label. Something that I corroborated with other samples prepared in house. This clearly affecting to determination of the reactivity of the antibody anti-GluT2 (ab95256) antibody, where 10 ug are recommended to detect the protein by Western blot.

    We have purchased twice this material and it has failed each time. Can we get a replacement of other pancreas lysate lot? If that is not the case what can we do?

    Read More
    Answer

    Thank you for your reply and also for checking the lysate. This is really helpful.

    I am sorry to hear that the problem has not been resolved.
    Since you see no protein bands before transfer, it does not surprise me that you see none after. I, too, think that the lysate should give you bands at 10 ug.

    I have checked what we have in stock for ab29816: It is only the same lot as you have tried.
    Also, this is the only human normal pancreas tissue whole cell lysate. All other human pancreas lysates are either disease-related or organelle specific (membrane, nuclear).

    If the antibody ab95256 works well with the liver lysate, I'd suggest to use this lysate as positive control.

    I'd be happy to credit or refund the purchases of the 2 vials of pancreas lysate ab29816 - unless you would like to replace them with another, differentlysate that we carry. Please let me know what would be best for you.

    I look forward to hear back and assist you in resolving this case.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    used liver and pancreas lysate (10 ug), lot GR26918-2
    liver lysate transfers well, pancreas lysate does not (but detects band when doing WB)
    ab85715
    Ab: 1/200, sees band in liver, but not in pancreas(coul dbe lysate issue - see above)

    suggested to stain gel before and after transfer with Coomassie blue, use more protein
    will check with lab regarding this lot

    Read More
    Answer

    Thank you for contacting us.
    I am sorry to hear about the problem the pancreas lysate is causing you.

    As we discussed over the phone, it would be worthwhile trying the following in order to determine the cause of the problem:

    1)stain gel before and after transfer with Coomassie blue (protein should be detected)
    2)use more protein (e.g. 10 ug, 20 ug or 50 ug) - if feasible

    I have also contacted the lab tocheckfor more information about this lotand additional suggestions. I will be in touch as soon as I hear back from them.

    I hope this information is of some help to you, and I look forward to hear back.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear sir or madame,
    I would like to know if you offer a biotinylated monoclonal Antibody anti mouse Glut2.
    If you do so please send me a proposition about 18mg of the requested Antibody.

    Read More
    Answer

    Vielen Dank für Ihre Anfrage.

    Leider haben wir keinen Antikörper, der allen genannten Anforderungen entspricht.

    Wir führen einen Maus-monoklonalen GLUT2-Antikörper, der bislang nur für humane Proben getestet wurde (ab85715). Das Immunogen zeigt eine Sequenzähnlichkeit von 81% gegenüber dem murinen GLUT2, und daher könnte der Antikörper mit Maus kreuzreagieren.
    ab85715:Click here (or use the following: https://www.abcam.com/Glucose-Transporter-GLUT2-antibody-5D1-ab85715.html).

    Daneben haben wir zwei GLUT2-Antikörper, die wir für die Anwendung in der Maus garantieren können (ab54460, ab104622). Jedoch sind die beiden polyklonale Antikörper aus dem Kaninchen.
    ab54460: Click here (or use the following: https://www.abcam.com/Glucose-Transporter-GLUT2-antibody-ab54460.html).
    ab104622: Click here (or use the following: https://www.abcam.com/Glucose-Transporter-GLUT2-antibody-ab104622.html).

    Leider ist auch keiner unserer GLUT2-Antikörper mit Biotin markiert. In der Regel würde ich daher einen unsere EasyLink-Konjugationskits empfehlen (ab102863 bzw. ab102866, https://www.abcam.com/EasyLink). Da Sie jedoch beträchtliche Mengen des Antikörpers benötigen, ist das sicher nicht praktikabel. Leider können wir derzeit auch keinen Konjugationsservice auf Anfrage für grössere Mengen anbieten.

    Zudem habe ich die Vermutung, dass Sie den Antikörper vielleicht für funktionelle Studien verwenden möchten, weil Sie nach 18 mg fragten. Leider ist unseres Wissens bislang keiner der GLUT2- Antikörper für so etwas getestet worden, und wir können daher nicht garantieren, dass es mit dem jeweiligen Produkt funktioniert. Wir geben alle getesteten Anwendungen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne Vorschläge zur Optimierung Ihres Protokolls oder gegebenenfalls einen Ersatz oder eine Gutschrift schicken.

    Aufgrund all dieser Unwägbarkeiten kommt vermutlich keiner der genannten Antikörper für Ihre Versuchsvorhaben in Betracht. Gerne möchte ich Ihnen daher die Suchmaschine BioCompare für Ihre weitere Suche empfehlen: http://www.biocompare.com

    Es tut mir leid, dass wir keine besseren Nachrichten für Sie haben, und ich hoffe, dass diese Angaben dennoch hilfreich für Sie sind. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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