Anti-Glucose Transporter GLUT1 抗体 (ab14683)

Thank you for your email.

All these antibodies are excellent in detecting GLUT1 by western blot and are fully guaranteed. I can however suggest using ab15309; https://www.abcam.com/Glucose-Transporter-GLUT1-antibody-ab15309.html
which reacts with rat and human cells. For other species please buy ab14683 or ab40084.

If any these products does not show the expected results. Please let us know we will be happy to help!

Thank you for contacting us.

Could you please explain what type of actualized data you would be interested in to see?

All three antibodies has been tested in WB and gave satisfactory results which is why WB application is guaranteed. We have sold 100s of units of these products and any positive or negative data submitted to us by our customers, is published under Abreviews and scientific support enquiries. These customers have successfully used these antibodies in western blot.

There are also many customer who reported observing multiple bands in western blot which is why we have updated the antibody datasheet with general note. We predict the multiple band or smear is probably due to high glycosylation or aggregation of protein due to heating. Our recommendations for best result are - not to heat the protein in sample buffer, 60-70C for 15-20 minutes will be OK and de-glycosylation.

These antibodies are tested for satisfactory results however if you are not convinced after using these products we will either replace the antibody or will provide you full refund.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Thank you for contacting us.
We always recommend our customers trying different procedure to get excellent results with antibodies sin IHC-P. It is a tricky applications due to antigen retrieval method involved.
Though citrate buffer pH 6.0 would be best however Tris-EDTA buffer can also be tried.
Microwave antigen retrieval procedure with a incubation time for 5, 10 and 15 minutes would be good to get the optimized time.
We have many IHC detection kits available. You can select any of these.
e.g. ab64264, ab93697, ab94698, ab93705, ab93677, ab64261, an64260 etc.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

I am so sorry for the long delay in our answer.

Indeed, the laboratory of one of the antibodies against GLUT1 was searching for an image, as they had performed the Western blot on erythrocyte ghost membranes about 10 years ago. However even after several reminders, they have not found it as is seems. I am very sorry for this. They were ableto confirm, that there will be a diffuseband on this sample.

I realize that you have might moved on meanwhile with your research. If however we could still be of any assistance, please do not hesitate to contact us again. Once more, I am really sorry for the delay in the answer.

I wish you a great week.

I am very pleased to hear you would like to accept our offer and test ab14683 on chicken samples. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for chicken and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for contacting Abcam.
For ab14683, we had not actually tested this antibody on chicken tissue, but based on sequence homology we thought there was a chance it would, hence the reason we said that it was predicted to work with this species.
I was wondering if you also ran a sample from mouse, rat or human as a positive control, as if you didn’t get a signal from that tissue, then we would know that there is something wrong with the vial we sent you. If however you got a signal from the mouse, rat or human, then we would know that this antibody is not recognizing the chicken sample.
Also, I was wondering how long you incubated your primary antibody for, was it overnight at 4C.
Looking forward to your reply.

Thank you for your phone call yesterday and for your patience while I have been in touch with the lab about ab14683. Based on the immunogen sequence, the antibody is predicted to react with chicken samples. However, since this has not been tested yet, we are unable to guarantee this cross-reactivity. We do have a testing discount program if you would like to try this antibody in chicken samples. You can purchase ab14683 at full price, test it in chicken, then submit an Abreview with your data. In exchange, you will receive one free primary antibody on a future purchase within 4 months of purchasing ab14683. If you are interested in this program, I will be happy to send more information. Please let me know if you have any questions, or if there is anything that we can do for you.

I have received the following recommendations from the laboratory: Extract cellular and membrane proteins in lysis buffer. The proteins were then dissolved in SDS-PAGE sample buffer 2X and reduced the in presence of 2.5-5% BME. All samples at this time will be heated 70-80oC for 3 minutes and aliquot and stored frozen till it is time for analysis. Just before analyses the samples much be thawed at room temp or at 370C to dissolve any precipitated SDS in the sample. The remaining sample must not be frozen again, this freeze and next thaw cycle will make aggregate in some samples. I hope this clarification helps.

I have received confirmation from the laboratory that tested ab14683 that this antibody labels Glut 1 protein as a smear due to large amount of glycosylation on Glut 1 transporter. We have also seen that boiling and freezing the Glut 1 samples resulted in an aggregated form of the protein and gives a smear form high 100kDa range to 45kDa with significant reduction in Glut 1 (44-47kDa) band. Based on these observations they have indeed suggested that samples to be analyzed should not be heated and frozen. I will add this information to our datasheet and hope this will also help you.