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AB91034

Anti-Glucose 6 Phosphate Dehydrogenase 抗体 [AT2F6]

Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6]

5

(2 Reviews)

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(3 Publications)

Mouse Monoclonal Glucose 6 Phosphate Dehydrogenase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glucose-6-phosphate 1-dehydrogenase aa 1 to C-terminus.

別名を表示する

Glucose-6-phosphate 1-dehydrogenase, G6PD

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (AB91034)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (AB91034)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Glucose 6 Phosphate Dehydrogenase with ab91034 at 1/100 dilution. The secondary antibody used was Alexa Fluor 488. DAPI was used to stain the cell nucleus (blue).

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (AB91034)
  • WB

Supplier Data

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (AB91034)

All lanes:

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (ab91034) at 1/1000 dilution

Lane 1:

MCF7 cell lysate

Lane 2:

HeLa cell lysate

Secondary

All lanes:

Goat Anti-Mouse HRP conjugated

Predicted band size: 59 kDa

true

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

AT2F6

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human Glucose-6-phosphate 1-dehydrogenase aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11413

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

This product was changed from ascites to tissue culture supernatant on 11th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 10% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.
Biological function summary

Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.

Pathways

Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.

Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
See full target information Glucose-6-phosphate 1-dehydrogenase
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:9333 PubMed37291185

2023

Primary mouse myoblast metabotropic purinoceptor profiles and calcium signalling differ with their muscle origin and are altered in mdx dystrophinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Justyna Róg,Aleksandra Oksiejuk,Dariusz C Górecki,Krzysztof Zabłocki

Cell metabolism 35:316-331.e6 PubMed36584675

2022

Metabolic adaptation supports enhanced macrophage efferocytosis in limited-oxygen environments.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ting Wang,Alissa J Trzeciak,Waleska Saitz Rojas,Pedro Saavedra,Yan-Ting Chen,Rachel Chirayil,Jon Iker Etchegaray,Christopher D Lucas,Daniel J Puleston,Kayvan R Keshari,Justin S A Perry

Nature communications 11:1533 PubMed32210228

2020

Subpopulation targeting of pyruvate dehydrogenase and GLUT1 decouples metabolic heterogeneity during collective cancer cell invasion.

Applications

Unspecified application

Species

Unspecified reactive species

R Commander,C Wei,A Sharma,J K Mouw,L J Burton,E Summerbell,D Mahboubi,R J Peterson,J Konen,W Zhou,Y Du,H Fu,M Shanmugam,A I Marcus
View all publications
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代替製品

Primary Antibodies

AB204473

Alexa Fluor® 647 Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (AB204473)

  • 5
  • 1
Primary Antibodies

AB133525

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (AB133525)

  • 5
  • 1
Primary Antibodies

AB124738

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291]

RabMAb|Recombinant

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291] (AB124738)

  • 0
  • 0
Primary Antibodies

AB210702

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

  • 3
  • 1
Primary Antibodies

AB231828

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)

  • 4
  • 6

組合せ製品

Assay Kits

AB102529

Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)

Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (AB102529)

  • 4
  • 1
Primary Antibodies

AB131263

Anti-IDH2 antibody [EPR7577]

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (AB131263)

  • 5
  • 1
Primary Antibodies

AB128568

Anti-CPT1A antibody [8F6AE9]

BOND RX™ Validated|KO Validated

Western blot - Anti-CPT1A antibody [8F6AE9] (AB128568)

  • 5
  • 5
Primary Antibodies

AB45174

Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (AB45174)

  • 4
  • 3
Primary Antibodies

AB115730

Anti-Glucose Transporter GLUT1 antibody [EPR3915]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB115730)

  • 4
  • 19

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