Anti-Glucocorticoid Receptor beta 抗体 (ab3581)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor beta
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Glucocorticoid Receptor beta antibody
Glucocorticoid Receptor beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Glucocorticoid Receptor beta -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Glucocorticoid Receptor beta aa 728-742.
Sequence:NVMWLKPESTSHTLI
(Peptide available asab39765) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3581の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa). Sample preparation: Lysates boiled in reducing buffer for 5 min. 20µg of protein loaded
Blocking agent: 5% non-fat milk in Tris buffered saline (TBS)
Primary antibody: overnight at 4°C
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特記事項 |
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WB
1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa). Sample preparation: Lysates boiled in reducing buffer for 5 min. 20µg of protein loaded Blocking agent: 5% non-fat milk in Tris buffered saline (TBS) Primary antibody: overnight at 4°C |
ターゲット情報
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機能
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation. -
組織特異性
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. -
関連疾患
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻訳後修飾
Increased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2908 Human
- SwissProt: P04150 Human
- Unigene: 122926 Human
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別名
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
画像
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Anti-Glucocorticoid Receptor beta antibody (ab3581) + hGR beta transfected COS-7 whole cell extract
Predicted band size: 83 kDa -
All lanes : Anti-Glucocorticoid Receptor beta antibody (ab3581) at 1/500 dilution
Lane 1 : COS-74 whole cell extract transfected with Glucocorticoid Receptor alpha
Lane 2 : COS-74 whole cell extract transfected with Glucocorticoid Receptor beta
Predicted band size: 83 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab3581 は 16 報の論文で使用されています。
- Chang HH et al. Novel Classification System of Adenoids Based on Appearance and Its Relationship with Drug Therapy. Int Arch Allergy Immunol 184:634-642 (2023). PubMed: 36996773
- Lo CY et al. Increased Interleukin-17 and Glucocorticoid Receptor-β Expression in Interstitial Lung Diseases and Corticosteroid Insensitivity. Front Immunol 13:905727 (2022). PubMed: 35865549
- Saif Z et al. A preferential switch between placental GR exon 1 promoter variants in the presence of maternal asthma or inflammation upregulates GRa D isoforms. Placenta 108:64-72 (2021). PubMed: 33819863
- Kolm I et al. Lichen aureus with pseudolymphomatous infiltrate. J Cutan Pathol 48:669-673 (2021). PubMed: 33368548
- Bernays V et al. Glucocorticoid Receptor Beta and Its Prognostic Value on Treatment Response in Chronic Vulvar Dermatitis. Skin Pharmacol Physiol 34:30-37 (2021). PubMed: 33592607