Anti-GLP-1 抗体 [4F3] (ab23472)

Thank you for contacting us.
I have had a look at the products that we have available in our catalogue and I recommend using the following validated antibody pairs for sandwich ELISA (sELISA):
1. Glucagon Like Peptide-1 (active)
I have found two different antibody pairs that have been validated for use in sELISA:
Capture: ab23472 (www.abcam.com/ab23472) and Detection: ab121080(www.abcam.com/ab121080).
or
Capture: ab26278 (www.abcam.com/ab26278) and Detection: ab121072(www.abcam.com/ab121072).
ab26278 is specific for the amidated C terminus of the peptide and does not react with GLP 1 (7-37). It reacts with the amidated C-terminus of GLP 1 (7-36) amide, GLP-1(9-36) and GLP 1 (1-36) amide.
2. Peptide YY
Capture: 121069 (www.abcam.com/ab121069) and Detection: ab121070(www.abcam.com/ab121070).
3. Human Ghrelin
Capture: 121066 (www.abcam.com/ab121066) and Detection: ab121067(www.abcam.com/ab121067).
Attached is a word document with the 4 “standard curves” from the 4 assays. Please note however that these assays have not been optimized and it is very likely that with optimization, you will be able to retrieve a better assay performance than what has been shown here.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Thank you for your inquiry. The peptide used as immunogen is corresponding to amino acids 7 to 36 of the human GLP1 as stated on the datasheet. The exact epitope this antibody was not further mapped and therefore not known. I hope this information is helpful and wish you good luck for your publication.

Thank you for contacting us. Indeed, we do not have an antibody tested for all the applications/species. We can however provide you with a testing discount if you want to test one of these antibodies against an untested species or application. In regards to know which one to chose, it depends also on your priorities. My comments on different anti GLP-1 antibodies are: -ab23468 - tested in rat, in ELISA, ICC and WB - As this antibody has been tested in applications with denatured epitope (WB), as well as native epitope (ELISA) I think has reasonable chances to provide good signal in IHC-P as well. Click here (or use the following: https://www.abcam.com/GLP1-antibody-11E2-ab23468.html). -ab23472 - is indeed tested in WB and IHC-P. The alignment of the immunogen with the rat protein is however not very good with 80% identity. As there is though a stretch of identical amino acids, it might work. Please see here the alignment. http://www.ebi.ac.uk/Tools/services/web/toolresult.ebi?jobId=clustalw2-I20110914-131516-0253-48328932-pg&tool=clustalw2&analysis=alignments -ab26278 - indeed this antibody will react with the rat protein in IHC. However it has not been tested in Western blot and as the epitope is linear in Western blot, it is not possible for me to make a prediction whether this antibody might work in Western blot. It would be necessary to assess this experimentally. I would therefore suggest the best option would be to test ab23468 in IHC-P. I can offer a discount off a future purchase if you buy ab23468 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. However if you prefer to test one of the other antibodies in rat or WB respectively, I could also provide you with the same testing offer. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab23468 in IHC-P (or what other antibody you would prefer to test). I will then send a discount code. This code must be issued before purchasing ab23468 so please wait for my reply before ordering. 2. Purchase ab23468 either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-P. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab23468 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.