Anti-GLO2 抗体 (ab154108)
Key features and details
- Rabbit polyclonal to GLO2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GLO2 antibody -
製品の詳細
Rabbit polyclonal to GLO2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow
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免疫原
Recombinant fragment corresponding to Human GLO2 aa 50-350.
Database link: Q16775 -
ポジティブ･コントロール
- HepG2 and Mouse liver whole cell lysates; Human NCI-N87 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154108の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Hydroxyacylglutathione hydrolase (HAGH) is a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. -
細胞内局在
Cytoplasmic and Mitochondrial -
参照データベース
- Entrez Gene: 509274 Cow
- Entrez Gene: 3029 Human
- Entrez Gene: 14651 Mouse
- Entrez Gene: 24439 Rat
- Omim: 138760 Human
- SwissProt: Q3B7M2 Cow
- SwissProt: Q16775 Human
- SwissProt: Q99KB8 Mouse
see all -
別名
- GLO2 antibody
- GLX II antibody
- Glyoxalase II antibody
see all
画像
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Western blot - Anti-GLO2 antibody (ab154108)Anti-GLO2 antibody (ab154108) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-GLO2 antibody (ab154108)Anti-GLO2 antibody (ab154108) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 29 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLO2 antibody (ab154108)Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling GLO2 with ab154108 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab154108 は 5 報の論文で使用されています。
- Li X et al. Generation of a GLO-2 deficient mouse reveals its effects on liver carbonyl and glutathione levels. Biochem Biophys Rep 28:101138 (2021). PubMed: 34584990
- Emidio GD et al. Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice. Cells 9:N/A (2020). PubMed: 31947651
- Di Emidio G et al. Regulatory Functions of L-Carnitine, Acetyl, and Propionyl L-Carnitine in a PCOS Mouse Model: Focus on Antioxidant/Antiglycative Molecular Pathways in the Ovarian Microenvironment. Antioxidants (Basel) 9:N/A (2020). PubMed: 32942589
- Di Emidio G et al. SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary. Biochim Biophys Acta Mol Basis Dis N/A:N/A (2019). WB ; Mouse . PubMed: 30771486
- Cordone V et al. Antiglycative Activity and RAGE Expression in Rett Syndrome. Cells 8:N/A (2019). PubMed: 30781346