Anti-Gliomedin 抗体 (ab24483)
Key features and details
- Rabbit polyclonal to Gliomedin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Gliomedin antibody -
製品の詳細
Rabbit polyclonal to Gliomedin -
由来種
Rabbit -
特異性
ab24483 detects a Gliomedin sized band at 70kDa in WB. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive signal in the following tissue lysates: Sciatic Nerve (Rat and Mouse) IF/ICC: PC12 cell line
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24483の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa). |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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関連性
Gliomedin is a member of the collagen superfamily, it is a glial ligand for neurofascin and NrCAM, two axonal immunoglobulin cell adhesion molecules that are associated with Na+ channels at the nodes of Ranvier. Gliomedin provides a glial cue for the formation of peripheral nodes of Ranvier. Gliomedin is expressed by myelinating Schwann cells and accumulates at the edges of each myelin segment during development, where it aligns with the forming nodes of ranvier. Eliminating the expression of gliomedin or the addition of a soluble extracellular domain of neurofascin to myelinating cultures abolishes node formation. Gliomedin is expressed in the PNS nodes of ranvier, but not in the CNS nodes of ranvier. Gliomedin also displays high expression in murine and human hepatocellular carcinomas (HCC). Its restricted expression in normal tissues and unique early upregulation during tumor development make it an excellent candidate as a new clinical marker of HCC. -
細胞内局在
Cell Membrane and Cytoplasmic -
参照データベース
- Entrez Gene: 342035 Human
- Entrez Gene: 235379 Mouse
- Entrez Gene: 315675 Rat
- Omim: 608603 Human
- SwissProt: Q6ZMI3 Human
- SwissProt: Q8BMF8 Mouse
- SwissProt: Q80WL1 Rat
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別名
- CANCER RELATED GENE LIVER 2 antibody
- Cancer related gene-Liver 2 antibody
- CLOM antibody
see all
画像
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Western blot - Anti-Gliomedin antibody (ab24483)All lanes : Anti-Gliomedin antibody (ab24483) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Tissue Lysate
Lane 2 : Rat Sciatic Nerve Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesab24483 detects a band of 70 kDa in Western Blot. Gliomedin contains a number of N-glycosylation sites (SwissProt data) which may explain the migration at a higher molecular weight than predicted based on primary sequence.
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Immunocytochemistry/ Immunofluorescence - Anti-Gliomedin antibody (ab24483)ICC/IF image of ab24483 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24483, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab24483 は 7 報の論文で使用されています。
- McGonigal R & Willison HJ The role of gangliosides in the organisation of the node of Ranvier examined in glycosyltransferase transgenic mice. J Anat 241:1259-1271 (2022). PubMed: 34605014
- McGonigal R et al. Schwann cell nodal membrane disruption triggers bystander axonal degeneration in a Guillain-Barré syndrome mouse model. J Clin Invest 132:N/A (2022). PubMed: 35671105
- Clark AJ et al. An iPSC model of hereditary sensory neuropathy-1 reveals L-serine-responsive deficits in neuronal ganglioside composition and axoglial interactions. Cell Rep Med 2:100345 (2021). PubMed: 34337561
- Kwon HS et al. Myocilin mediates myelination in the peripheral nervous system through ErbB2/3 signaling. J Biol Chem 288:26357-71 (2013). PubMed: 23897819
- Kawamura N et al. Anti-neurofascin antibody in patients with combined central and peripheral demyelination. Neurology 81:714-22 (2013). Human . PubMed: 23884033
- Thaxton C et al. Nodes of Ranvier Act as Barriers to Restrict Invasion of Flanking Paranodal Domains in Myelinated Axons. Neuron 69:244-57 (2011). PubMed: 21262464
- Thaxton C et al. In vivo deletion of immunoglobulin domains 5 and 6 in neurofascin (Nfasc) reveals domain-specific requirements in myelinated axons. J Neurosci 30:4868-76 (2010). PubMed: 20371806