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Anti-Gliadin 抗体 [14D5] (ab36729)

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Western blot - Anti-Gliadin antibody [14D5] (ab36729)

    Key features and details

    • Mouse monoclonal [14D5] to Gliadin
    • Suitable for: WB
    • Reacts with: Wheat, Hordeum vulgare
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-Gliadin antibody [14D5]

    • 製品の詳細

      Mouse monoclonal [14D5] to Gliadin

    • 由来種

      Mouse

    • 特異性

      This antibody reacts specifically with the deamidated peptide (KLQPFPQPELPYPQPQ) when coated directly in an immunoplate. Does not react with Oats. This antibody reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Wheat, Hordeum vulgare, Secale cereale

    • 免疫原

      Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)]

      KLQPFPQPELPYPQPQ

      ) coupled to immunogenic carrier protein.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • エピトープ

      The epitope is located in the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide.

    • 特記事項


      The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • バッファー

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituents: 2.9% Sodium chloride, 0.0268% PBS
    • Concentration information loading...

    • 精製度

      Protein A purified

    • 一次抗体 備考

      The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.

    • ポリ/モノ

      モノクローナル

    • クローン名

      14D5

    • ミエローマ

      x63-Ag8.653

    • アイソタイプ

      IgG2a

    • 軽鎖の種類

      kappa

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab36729の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/1000.

    dilution 1/1000
    特記事項
    WB
    1/1000.

    dilution 1/1000

    ターゲット情報

    • 関連性

      Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The alcohol soluble proteins (prolamins) from wheat, rye, barley and oats produce the harmful effect of coeliac disease or gluten sensitive enteropathy in humans by causing characteristic changes in the intestinal mucosa. Patients so affected must avoid eating these grains and replace them with rice, corn, potatoes, etc. Many gluten-free foods are produced industrially, thus several immunoassays have been developed for determination of gliadin in supposedly gluten-free foods.

    • 細胞内局在

      Cytoplasmic; major seed storage protein in wheat.

    • 参照データベース

      see all

    • 別名

      • prolamin antibody

    画像

    • Western blot - Anti-Gliadin antibody [14D5] (ab36729)
      Western blot - Anti-Gliadin antibody [14D5] (ab36729)
      All lanes : Anti-Gliadin antibody [14D5] (ab36729) at 1/1000 dilution

      Lane 1 : Rye (Secale cereale) Lysate
      Lane 2 : Barley (Hordeum vulgare) Lysate
      Lane 3 : Wheat Lysate

      Performed under reducing conditions.

    参考文献 (4)

    ab36729 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab36729 は 4 報の論文で使用されています。

    • Lee GY  et al. Identification and Growth Characteristics of a Gluten-Degrading Bacterium from Wheat Grains for Gluten-Degrading Enzyme Production. Microorganisms 11:N/A (2023). PubMed: 38138028
    • Méndez PX  et al. The Ability of the Yeast Wickerhamomyces anomalus to Hydrolyze Immunogenic Wheat Gliadin Proteins. Foods 11:N/A (2022). PubMed: 36553848
    • Manai F  et al. The In Vitro Effects of Enzymatic Digested Gliadin on the Functionality of the Autophagy Process. Int J Mol Sci 19:N/A (2018). ICC/IF . PubMed: 29473905
    • Engstrom N  et al. Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl2 as detoxifiers. Sci Rep 7:77 (2017). PubMed: 28250436

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