Anti-Gli2 抗体 [OTI1F9] (ab187386)
![Western blot - Anti-Gli2 antibody [OTI1F9] (ab187386)](https://www.abcam.co.jp/ps/products/187/ab187386/Images/ab187386-219653-anti-gli2-antibody-oti1f9-western-blot.jpg "Western blot - Anti-Gli2 antibody [OTI1F9] (ab187386)")
Key features and details
- Mouse monoclonal [OTI1F9] to Gli2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Gli2 antibody [OTI1F9]
Gli2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI1F9] to Gli2 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Gli2 aa 700-1150. Produced in E.coli. (NP_005261).
Database link: P10070-5 -
ポジティブ･コントロール
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY Gli2 cDNA. IHC-P: Human skin tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Purified from cell culture supernatant by affinity chromatography -
ポリ/モノ
モノクローナル -
クローン名
OTI1F9 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187386の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500. Predicted molecular weight: 167 kDa.
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| IHC-P |
1/150.
Perform heat mediated antigen retrieval with Tris-EDTA, pH8.0 before commencing with IHC staining protocol. |
| 特記事項 |
|---|
|
WB
1/500. Predicted molecular weight: 167 kDa. |
|
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris-EDTA, pH8.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal. -
関連疾患
Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. -
配列類似性
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
翻訳後修飾
Phosphorylated in vitro by ULK3. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2736 Human
- Omim: 165230 Human
- SwissProt: P10070 Human
- Unigene: 111867 Human
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別名
- CJS antibody
- Gli 2 antibody
- GLI family zinc finger 2 antibody
see all
画像
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Western blot - Anti-Gli2 antibody [OTI1F9] (ab187386)All lanes : Anti-Gli2 antibody [OTI1F9] (ab187386) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY Gli2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 167 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli2 antibody [OTI1F9] (ab187386)](https://www.abcam.co.jp/ps/products/187/ab187386/Images/ab187386-219651-anti-gli2-antibody-oti1f9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli2 antibody [OTI1F9] (ab187386)Paraffin-embedded Human skin tissue labeling Gli2 with ab187386 at 1/150 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab187386 は 7 報の論文で使用されています。
- Na L et al. MAML3 Contributes to Induction of Malignant Phenotype of Gallbladder Cancer Through Morphogenesis Signalling Under Hypoxia. Anticancer Res 43:2909-2922 (2023). PubMed: 37351966
- Liu X et al. MUC21 controls melanoma progression via regulating SLITRK5 and hedgehog signaling pathway. Cell Biol Int 46:1458-1467 (2022). PubMed: 35579188
- Ichimiya S et al. GLI2 but not GLI1/GLI3 plays a central role in the induction of malignant phenotype of gallbladder cancer. Oncol Rep 45:997-1010 (2021). PubMed: 33650666
- Peng Y et al. Dual activation of Hedgehog and Wnt/ß-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer. Aging (Albany NY) 13:10749-10769 (2021). PubMed: 33848981
- Ye G et al. Circ_DCAF6 potentiates cell stemness and growth in breast cancer through GLI1-Hedgehog pathway. Exp Mol Pathol 116:104492 (2020). PubMed: 32668286
- Hua J et al. Small Molecule-Based Strategy Promotes Nucleus Pulposus Specific Differentiation of Adipose-Derived Mesenchymal Stem Cells. Mol Cells 42:661-671 (2019). PubMed: 31564076
- Zhang CY et al. Saikosaponin-d Inhibits the Hepatoma Cells and Enhances Chemosensitivity Through SENP5-Dependent Inhibition of Gli1 SUMOylation Under Hypoxia. Front Pharmacol 10:1039 (2019). PubMed: 31616295