AB277800

Anti-Gli2 抗体 [9HCLC]

Anti-Gli2 antibody [9HCLC]

Recombinant
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(5 Publications)

Rabbit Recombinant Multiclonal GLI2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLI2 aa 1-350.

別名を表示する

THP, GLI2, Zinc finger protein GLI2, GLI family zinc finger protein 2, Tax helper protein

1 Images

Supplier Data

Western blot - Anti-Gli2 antibody [9HCLC] (AB277800)

All lanes:

Western blot - Anti-Gli2 antibody [9HCLC] (ab277800) at 2 µg/mL

Lane 1:

MDA-MB-231 nuclear enriched extracts at 30 µg

Lane 2:

MDA-MB-231 treated with TGF-β1 (Serum starved and treated with 5 ng/mL for 48hr) nuclear enriched extracts at 30 µg

Lane 3:

MCF-7 nuclear enriched extracts at 30 µg

Lane 4:

MCF-7 treated with TGF-β1 (Serum starved and treated with 5 ng/mL for 48hr) nuclear enriched extracts at 30 µg

Lane 5:

A549 nuclear enriched extracts at 30 µg

Lane 6:

A549 treated with TGF-β1 (Serum starved and treated with 5 ng/mL for 48hr) nuclear enriched extracts at 30 µg

Lane 7:

Mouse pancreas tissue extract at 30 µg

Lane 8:

Rat Pancreas tissue extract at 30 µg

Lane 9:

Mouse Kidney tissue extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/2500 dilution

Predicted band size: 167 kDa

false

Key facts

宿主種

Rabbit

クローン性

Multiclonal

クローン番号

9HCLC

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Mouse, Human

アプリケーション

applications

免疫原

Recombinant Fragment Protein within Human GLI2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P10070

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" } } }

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製品の詳細

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Gli2 also known as GLI family zinc finger 2 is a transcription factor with a molecular mass of approximately 160 kDa. It is an important component of the Hedgehog (Hh) signaling pathway. Gli2 primarily resides in the cytoplasm and migrates to the nucleus upon activation. Expression of Gli2 is widespread but most notable in developing tissues and adult brain. In the nucleus it regulates transcription by binding to specific DNA sequences.

Biological function summary

Gli2 acts as an activator or repressor of gene expression depending on the cellular context. It forms part of the Gli family of transcription factors playing a significant role in cellular growth differentiation and tissue patterning. While not usually part of stable protein complexes Gli2 interacts transiently with other proteins such as Suppressor of Fused (SuFu) to regulate its transcriptional activity. This interaction influences the processing and degradation of Gli proteins.

Pathways

The Hedgehog signaling cascade prominently features Gli2 as a critical mediator. This pathway is fundamental in embryonic development. Alongside other Gli proteins like Gli1 and Gli3 Gli2 helps to translate extracellular Hh signals into precise cellular responses. In the absence of Hh ligands Gli2 remains tethered in the cytoplasm in a complex with SuFu limiting its transcription activity. Upon Hh pathway activation Gli2 translocates to the nucleus and modulates target gene expression.

Gli2 has a notable connection to cancers including basal cell carcinoma and medulloblastoma. Overactive Hedgehog signaling often involves dysregulation of Gli2 which promotes tumorigenesis by driving inappropriate cellular proliferation. Additionally Gli2 links to the genetic disorder Greig cephalopolysyndactyly syndrome via its interactions with Gli3 protein. These connections underline the importance of Gli2 as a therapeutic target in certain pathological conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Functions as a transcription regulator in the hedgehog (Hh) pathway (PubMed : 18455992, PubMed : 26565916). Functions as a transcriptional activator (PubMed : 19878745, PubMed : 24311597, PubMed : 9557682). May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed : 15994174, PubMed : 20685856).. Isoform 1. Involved in the smoothened (SHH) signaling pathway.. Isoform 2. Involved in the smoothened (SHH) signaling pathway.. Isoform 3. Involved in the smoothened (SHH) signaling pathway.. Isoform 4. Involved in the smoothened (SHH) signaling pathway.. Isoform 1. Acts as a transcriptional activator in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed : 9557682).. Isoform 2. (Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed : 9557682).. Isoform 3. (Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed : 9557682).. Isoform 4. (Microbial infection) Acts as a transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1 (PubMed : 9557682).. Isoform 5. Acts as a transcriptional repressor.

See full target information GLI2

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1209 PubMed40804340

2025

rTMS ameliorates cerebral ischemia-reperfusion injury by inhibiting Golgi apparatus stress through epigenetic modulation of Gli2.

Applications

Unspecified application

Species

Unspecified reactive species

Chunjiao Zhu,Yongmei Fan,Wenna Peng

Cell reports. Medicine 5:101700 PubMed39208799

2024

Gemcitabine therapeutically disrupts essential SIRT1-mediated p53 repression in atypical teratoid/rhabdoid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Dennis S Metselaar,Michaël H Meel,Joshua R Goulding,Aimeé du Chatinier,Leyla Rigamonti,Piotr Waranecki,Neal Geisemeyer,Mark C de Gooijer,Marjolein Breur,Jan Koster,Sophie E M Veldhuijzen van Zanten,Marianna Bugiani,Niels E Franke,Alyssa Reddy,Pieter Wesseling,Gertjan J L Kaspers,Esther Hulleman

Genetics and molecular biology 47:e20230347 PubMed38985013

2024

ΔNp63α promotes cigarette smoke-induced renal cancer stem cell activity via the Sonic Hedgehog pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiang Zhao,Nannan Ma,Wanngyu Wu,Ying Wu,Wenbo Zhang,Weiwei Qian,Xin Sun,Tao Zhang

Respiratory research 23:86 PubMed35395850

2022

C/EBP homologous protein promotes Sonic Hedgehog secretion from type II alveolar epithelial cells and activates Hedgehog signaling pathway of fibroblast in pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Yang,Wei Sun,Xiaoyan Jing,Qian Zhang,Hui Huang,Zuojun Xu

Oncology reports 46: PubMed34278506

2021

miR‑367‑3p downregulates Rab23 expression and inhibits Hedgehog signaling resulting in the inhibition of the proliferation, migration, and invasion of prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Du,Dong Li,Jianhao Xie,Ping Tang

View all publications

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Abcam product promise

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Anti-Gli2 抗体 [9HCLC]

Anti-Gli2 antibody [9HCLC]

Western blot - Anti-Gli2 antibody [9HCLC] (AB277800)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise