Anti-GIV 抗体 (ab113890)
Key features and details
- Rabbit polyclonal to GIV
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-GIV antibody
GIV 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GIV -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human GIV conjugated to keyhole limpet haemocyanin.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113890の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa.
|
|
| IHC-P |
Use a concentration of 5 µg/ml.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa. |
|
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
-
機能
Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. -
組織特異性
Expressed ubiquitously. -
配列類似性
Belongs to the CCDC88 family. -
翻訳後修飾
Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration. -
細胞内局在
Membrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Localizes to the cell membrane through interaction with phosphoinositides. - Information by UniProt
-
参照データベース
- Entrez Gene: 55704 Human
- Entrez Gene: 108686 Mouse
- Entrez Gene: 305605 Rat
- Omim: 609736 Human
- SwissProt: Q3V6T2 Human
- SwissProt: Q5SNZ0 Mouse
- Unigene: 292925 Human
- Unigene: 338284 Mouse
see all -
別名
- AKT iphosphorylation enhancer antibody
- Akt phosphorylation enhancer antibody
- APE antibody
see all
画像
-
Western blot - Anti-GIV antibody (ab113890)Lane 1 : Anti-GIV antibody (ab113890) at 1 µg/ml
Lane 2 : Anti-GIV antibody (ab113890) at 2 µg/ml
All lanes : Rat brain lysate
Predicted band size: 216 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIV antibody (ab113890)ab113890, at 5 µg/ml, staining GIV in Formalin-Fixed, Paraffin-Embedded Human tonsil tissue by immunohistochemistry.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab113890 は 4 報の論文で使用されています。
- Zhang Y et al. Prognosis of gastric adenocarcinoma associated with girdin, Akt, and cortactin. Ann Saudi Med 42:181-190 (2022). PubMed: 35770962
- Dang J et al. Roles of metformin-mediated girdin expression in metastasis of epithelial ovarian cancer. Cell Biol Int 45:1030-1037 (2021). PubMed: 33404163
- Lan Y et al. Long noncoding RNA MEG3 prevents vascular endothelial cell senescence by impairing miR-128-dependent Girdin downregulation. Am J Physiol Cell Physiol 316:C830-C843 (2019). PubMed: 30576236
- Yang Z et al. Girdin protein: A potential metastasis predictor associated with prognosis in lung cancer. Exp Ther Med 15:2837-2843 (2018). PubMed: 29456687