Anti-GIT1 抗体 (ab153958)
Key features and details
- Rabbit polyclonal to GIT1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GIT1 antibody
GIT1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GIT1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human GIT1 aa 375-631 (internal sequence).
Database link: Q9Y2X7 -
ポジティブ･コントロール
- IHC-P: Human mixed ovarian cancer. ICC/IF: HeLa cells. WB: HepG2 whole cell lysate. Rat and mouse brain tissue lysate. HUVEC cell extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153958の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 84 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 84 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. -
配列類似性
Contains 3 ANK repeats.
Contains 1 Arf-GAP domain. -
ドメイン
The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6. -
翻訳後修飾
Phosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase. -
細胞内局在
Cytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin. - Information by UniProt
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参照データベース
- Entrez Gene: 28964 Human
- Entrez Gene: 216963 Mouse
- Entrez Gene: 83709 Rat
- Omim: 608434 Human
- SwissProt: Q9Y2X7 Human
- SwissProt: Q68FF6 Mouse
- SwissProt: Q9Z272 Rat
- Unigene: 514051 Human
see all -
別名
- ARF GAP GIT1 antibody
- ARF GTPase activating protein GIT1 antibody
- ARF GTPase-activating protein GIT1 antibody
see all
画像
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Western blot - Anti-GIT1 antibody (ab153958)Anti-GIT1 antibody (ab153958) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 84 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GIT1 antibody (ab153958)Immunofluorescence analysis of methanol-fixed HeLa labeling GIT1 with ab153958 at 1/500 dilution. Blue: Costaining with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIT1 antibody (ab153958)Immunohistochemical analysis of paraffin-embedded Human mixed ovarian cancer tissue labeling GIT1 with ab153958 at 1/100 dilution.
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Western blot - Anti-GIT1 antibody (ab153958)All lanes : Anti-GIT1 antibody (ab153958) at 1/1000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 84 kDa7.5% SDS-PAGE
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Western blot - Anti-GIT1 antibody (ab153958)Anti-GIT1 antibody (ab153958) at 1/500 dilution + HUVEV (Human umbilical vein endothelial cell line) whole cell extract at 30 µg
Predicted band size: 84 kDa7.5% SDS-PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab153958 は 2 報の論文で使用されています。
- Yang S et al. Decreased long non-coding RNA lincFOXF1 indicates poor progression and promotes cell migration and metastasis in osteosarcoma. J Cell Mol Med 24:12633-12641 (2020). PubMed: 32945076
- Ye X & Chen X miR-149-5p inhibits cell proliferation and invasion through targeting GIT1 in medullary thyroid carcinoma. Oncol Lett 17:372-378 (2019). PubMed: 30655777