Anti-GHRHR 抗体 (ab76263)
Key features and details
- Rabbit polyclonal to GHRHR
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GHRHR antibody
GHRHR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GHRHR -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human GHRHR (C terminal).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76263の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 47 kDa. Use BSA for blocking
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa. Use BSA for blocking |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. -
組織特異性
Pituitary gland. -
関連疾患
Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. -
配列類似性
Belongs to the G-protein coupled receptor 2 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2692 Human
- Omim: 139191 Human
- SwissProt: Q02643 Human
- Unigene: 767 Human
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別名
- GHRFR antibody
- GHRH R antibody
- GHRH receptor antibody
see all
画像
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Western blot - Anti-GHRHR antibody (ab76263)All lanes : Anti-GHRHR antibody (ab76263) at 1/500 dilution
Lane 1 : Extracts from LOVO cells
Lane 2 : Extracts from LOVO cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: >117 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-GHRHR antibody (ab76263)Immunofluorescence analysis of HUVEC cells using ab76263 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-GHRHR antibody (ab76263)Image from Vakili H et al., Molecular and Cellular Endocrinology: Volume 345, Issues 1–2, 15 October 2011, Pages 48–57. Fig 2.; doi: 10.1016/j.mce.2011.07.010. Epub 2011 Jul 12.Immunofluorescence analysis of mouse pituitary cells, staining GHRHR with ab76263.
Cells were fixed with 4% paraformaldehyde and blocked using 4% NGS in PBS with 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1/50) in 4% NGS for 18 hours at 4°C. Slides were washed three times for 15 min with PBS and then incubated in 4% NGS with an AlexaFluor®488-conjugated anti- rabbit secondary antibody (1/1000) for 1 hour at room temperature.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab76263 は 21 報の論文で使用されています。
- Du L et al. Growth hormone releasing hormone signaling promotes Th17 cell differentiation and autoimmune inflammation. Nat Commun 14:3298 (2023). PubMed: 37280225
- Granato G et al. Growth hormone-releasing hormone antagonist MIA-602 inhibits inflammation induced by SARS-CoV-2 spike protein and bacterial lipopolysaccharide synergism in macrophages and human peripheral blood mononuclear cells. Front Immunol 14:1231363 (2023). PubMed: 37649486
- Gesmundo I et al. Antagonists of Growth Hormone-Releasing Hormone Inhibit the Growth of Pituitary Adenoma Cells by Hampering Oncogenic Pathways and Promoting Apoptotic Signaling. Cancers (Basel) 13:N/A (2021). PubMed: 34439107
- Cong Z et al. Constitutive signal bias mediated by the human GHRHR splice variant 1. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34599099
- Recinella L et al. Antinflammatory, antioxidant, and behavioral effects induced by administration of growth hormone-releasing hormone analogs in mice. Sci Rep 10:732 (2020). PubMed: 31959947