Anti-GGT7 抗体 [EPR24550-76] (ab273046)
![Western blot - Anti-GGT7 antibody [EPR24550-76] (ab273046)](https://www.abcam.co.jp/ps/products/273/ab273046/Images/ab273046-482636-AntiGGT7antibodyEPR2455076westernblot1.jpg "Western blot - Anti-GGT7 antibody [EPR24550-76] (ab273046)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24550-76] to GGT7
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GGT7 antibody [EPR24550-76]
GGT7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24550-76] to GGT7 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human hypothalamus lysate lysate, U-87 MG whole cell lysate, U-2 OS transfected with scrambled siRNA control.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24550-76 -
アイソタイプ
IgG -
研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab273046の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 110,90,70 kDa (predicted molecular weight: 70 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 110,90,70 kDa (predicted molecular weight: 70 kDa). |
追加情報
Is unsuitable for Flow Cyt or IHC-P.
ターゲット情報
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関連性
GGT7 (Gamma-glutamyltransferase 7) is a member of a gene family that encodes enzymes involved in both the metabolism of glutathione and in the transpeptidation of amino acids. Changes in the activity of gamma glutamyltransferase may signal preneoplastic or toxic conditions in the liver or kidney. GGT7 consists of a heavy and a light chain, and it can interact with CT120, a plasma membrane associated protein that is possibly involved in lung carcinogenesis. -
細胞内局在
Membrane; Single pass type II membrane protein. -
参照データベース
- Entrez Gene: 2686 Human
- Omim: 612342 Human
- SwissProt: Q9UJ14 Human
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別名
- D20S101 antibody
- dJ18C9.2 antibody
- Gamma glutamyltransferase 4 antibody
see all
画像
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Western blot - Anti-GGT7 antibody [EPR24550-76] (ab273046)All lanes : Anti-GGT7 antibody [EPR24550-76] (ab273046) at 1/1000 dilution
Lane 1 : Human hypothalamus lysate
Lane 2 : Human hypothalamus treated with PNGase F lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 4 : U-87 MG treated with PNGase F lysate, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Additional bands at: 110 kDa (possible glycosylated form), 90 kDa (possible glycosylated form)
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-GGT7 antibody [EPR24550-76] (ab273046)](https://www.abcam.co.jp/ps/products/273/ab273046/Images/ab273046-482638-AntiGGT7antibodyEPR2455076westernblot2.jpg)
Western blot - Anti-GGT7 antibody [EPR24550-76] (ab273046)All lanes : Anti-GGT7 antibody [EPR24550-76] (ab273046)
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : U-2 OS transfected with GTT7-siRNA, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was developed using a higher sensitivity ECL substrate.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-GGT7 antibody [EPR24550-76] (ab273046)](https://www.abcam.co.jp/ps/products/273/ab273046/Images/ab273046-482662-Benefits-of-Recombinant-antibodies.jpg)
Anti-GGT7 antibody [EPR24550-76] (ab273046)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab273046 は論文での使用が確認できていません。