Anti-GGA2 抗体 [EPR14895] (ab185557)
![Western blot - Anti-GGA2 antibody [EPR14895] (ab185557)](https://www.abcam.co.jp/ps/products/185/ab185557/Images/ab185557-220188-anti-gga2-antibody-epr14895-western-blot.jpg "Western blot - Anti-GGA2 antibody [EPR14895] (ab185557)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14895] to GGA2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GGA2 antibody [EPR14895]
GGA2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14895] to GGA2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Jurkat and Raji cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14895 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185557の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
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| 特記事項 |
|---|
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WB
1/1000 - 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
ターゲット情報
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機能
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain. -
ドメイン
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function. -
翻訳後修飾
Ubiquitinated. -
細胞内局在
Golgi apparatus > trans-Golgi network membrane. Endosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 23062 Human
- Entrez Gene: 293455 Rat
- Omim: 606005 Human
- SwissProt: Q9UJY4 Human
- Unigene: 460336 Human
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別名
- 1200007E24Rik antibody
- ADP ribosylation factor binding protein 2 antibody
- ADP ribosylation factor binding protein GGA 2 antibody
see all
画像
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Western blot - Anti-GGA2 antibody [EPR14895] (ab185557)All lanes : Anti-GGA2 antibody [EPR14895] (ab185557) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa -
![Anti-GGA2 antibody [EPR14895] (ab185557)](https://www.abcam.co.jp/ps/products/185/ab185557/Images/ab185557-2-benefits-of-recombinant-antibodies.png)
Anti-GGA2 antibody [EPR14895] (ab185557)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab185557 は 1 報の論文で使用されています。
- O'Farrell H et al. Integrative Genomic Analyses Identifies GGA2 as a Cooperative Driver of EGFR-Mediated Lung Tumorigenesis. J Thorac Oncol 14:656-671 (2019). PubMed: 30578931