AB125069

Anti-GFPT1 抗体 [EPR4854]

Name: Anti-GFPT1 抗体 [EPR4854] (ab125069) | Abcam
Brand: Abcam Limited
SKU: AB125069
Price: 61000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-GFPT1 antibody [EPR4854]

RabMAb
Recombinant
詳細を見る

(2 Reviews)

(29 Publications)

Rabbit Recombinant Monoclonal GFPT1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 29 publications.

別名を表示する

GFAT, GFPT, GFPT1, Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1, D-fructose-6-phosphate amidotransferase 1, Glutamine:fructose-6-phosphate amidotransferase 1, Hexosephosphate aminotransferase 1, GFAT 1, GFAT1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854] (AB125069)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854] (AB125069)

ab125069, at a 1/100 dilution, staining GFPT1 in paraffin-embedded Human testis tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854] (AB125069)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling GFPT1 with Purified ab125069 at 1 : 1000 dilution (2.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Unknown

Immunoprecipitation - Anti-GFPT1 antibody [EPR4854] (AB125069)

ab125069 (purified) at 1 : 100 dilution (2μg) immunoprecipitating GFPT1 in Jurkat whole cell lysate.
Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+) : ab125069 & Jurkat whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab125069 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-GFPT1 antibody [EPR4854] (ab125069)

Predicted band size: 33 kDa,73 kDa,79 kDa,82 kDa

Observed band size: 60 kDa

false

Lab

Western blot - Anti-GFPT1 antibody [EPR4854] (AB125069)

This antibody is not suitable to detect tissue lysate samples.

All lanes:

Western blot - Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Mouse heart lysates at 20 µg

Lane 3:

Mouse cerebral cortex lysates at 20 µg

Lane 4:

MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates at 20 µg

Lane 5:

Rat heart lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

Lab

Western blot - Anti-GFPT1 antibody [EPR4854] (AB125069)

All lanes:

Western blot - Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/200 dilution

All lanes:

C6 (Rat glial tumor glial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

Unknown

Western blot - Anti-GFPT1 antibody [EPR4854] (AB125069)

All lanes:

Western blot - Anti-GFPT1 antibody [EPR4854] (ab125069) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Human placenta lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 79 kDa

true

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4854

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "For unpurified use at 1/1000 - 1/1000." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "For unpurified use at 1/1000 - 1/1000." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "For unpurified use at 1/1000 - 1/1000." } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes BMAL1 and CRY1 (By similarity). Has a role in fine tuning the metabolic fluctuations of cytosolic UDP-GlcNAc and its effects on hyaluronan synthesis that occur during tissue remodeling (PubMed : 26887390).

See full target information GFPT1

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文献 (29)

Recent publications for all applications. Explore the full list and refine your search

Research (Washington, D.C.) 8:0809 PubMed40740652

2025

Combinational Analysis of Metabolomic and O-GlcNAcylation Omics Reveals the HBP Metabolic Regulation of Chemoresistance via GFPT1/NR3C1 O-GlcNAcylation/GPX4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Zhou,Chunlin Zhang,Li Li,Zhenwei Feng,Xuesong Bai,Yuhua Mei,Weiyang He,Xin Gou,Xinyuan Li

International journal of molecular sciences 26: PubMed40141354

2025

O-GlcNAcylation in Gli1 Mesenchymal Stem Cells Is Indispensable for Bone Formation and Fracture Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Moyu Liu,Yujie Hu,Chengjia You,Ding Xiong,Ling Ye,Yu Shi

EMBO reports 25:4465-4487 PubMed39256595

2024

O-GlcNAcylation mediates Wnt-stimulated bone formation by rewiring aerobic glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Chengjia You,Fangyuan Shen,Puying Yang,Jingyao Cui,Qiaoyue Ren,Moyu Liu,Yujie Hu,Boer Li,Ling Ye,Yu Shi

Disease models & mechanisms 17: PubMed38903011

2024

Muscle-specific lack of Gfpt1 triggers ER stress to alleviate misfolded protein accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ruchen Zhang,Paniz Farshadyeganeh,Bisei Ohkawara,Kazuki Nakajima,Jun-Ichi Takeda,Mikako Ito,Shaochuan Zhang,Yuki Miyasaka,Tamio Ohno,Madoka Mori-Yoshimura,Akio Masuda,Kinji Ohno

iScience 26:107746 PubMed37744035

2023

Splicing regulation of GFPT1 muscle-specific isoform and its roles in glucose metabolisms and neuromuscular junction.

Applications

Unspecified application

Species

Unspecified reactive species

Paniz Farshadyeganeh,Mohammad Nazim,Ruchen Zhang,Bisei Ohkawara,Kazuki Nakajima,Mohammad Alinoor Rahman,Farhana Nasrin,Mikako Ito,Jun-Ichi Takeda,Kenji Ohe,Yuki Miyasaka,Tamio Ohno,Akio Masuda,Kinji Ohno

Cell reports methods 3:100518 PubMed37533645

2023

Enzymatic assay for UDP-GlcNAc and its application in the parallel assessment of substrate availability and protein O-GlcNAcylation.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Sunden,Divya Upadhyay,Rishi Banerjee,Nina Sipari,Vineta Fellman,Jukka Kallijärvi,Janne Purhonen

Cells 12: PubMed36980207

2023

Astragalus Polysaccharide Promotes Doxorubicin-Induced Apoptosis by Reducing O-GlcNAcylation in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhe Li,Fangfang Duan,Zhiqiang Pan,Xiaomei Liu,Wenli Lu,Chao Liang,Zhaoqin Fang,Peike Peng,Dongwei Jia

The Journal of biological chemistry 298:102437 PubMed36041631

2022

The RNA-binding protein AUF1 facilitates Akt phosphorylation at the membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Ling Li,Aparna Ragupathi,Nikhil Patel,Tatiana Hernandez,Jedrick Magsino,Guy Werlen,Gary Brewer,Estela Jacinto

Cell discovery 8:77 PubMed35945223

2022

GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation.

Applications

Unspecified application

Species

Unspecified reactive species

Shupei Wei,Qin Zhao,Ke Zheng,Peiying Liu,Nannan Sha,Yingzi Li,Chunmin Ma,Jingjie Li,Lingang Zhuo,Guanxin Liu,Wenhua Liang,Yuhui Jiang,Tao Chen,Nanshan Zhong

Life science alliance 5: PubMed35396334

2022

The hexosamine pathway and coat complex II promote malignant adaptation to nutrient scarcity.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Dragic,Audrey Barthelaix,Cédric Duret,Simon Le Goupil,Hadrien Laprade,Sophie Martin,Sabine Brugière,Yohann Couté,Christelle Machon,Jerome Guitton,Justine Rudewicz,Paul Hofman,Serge Lebecque,Cedric Chaveroux,Carole Ferraro-Peyret,Toufic Renno,Serge N Manié

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GFPT1 抗体 [EPR4854]

Anti-GFPT1 antibody [EPR4854]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854] (AB125069)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFPT1 antibody [EPR4854] (AB125069)

Immunoprecipitation - Anti-GFPT1 antibody [EPR4854] (AB125069)

Western blot - Anti-GFPT1 antibody [EPR4854] (AB125069)

Western blot - Anti-GFPT1 antibody [EPR4854] (AB125069)

Western blot - Anti-GFPT1 antibody [EPR4854] (AB125069)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (29)

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