Anti-GFP 抗体 [E385]

• WB

Lab

Western blot - Anti-GFP antibody [E385] (AB32146)

Blocking and dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution

Lane 1:

HeLa whole cell lysate (negative control)

Lane 2:

HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.001 µg

Lane 3:

HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.002 µg

Lane 4:

HeLa whole cell lysate spike with recombinant proprietary tag-GFP fusion protein at 0.0025 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

• WB

Supplier Data

Western blot - Anti-GFP antibody [E385] (AB32146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PADI1, PADI3, PADI4 and PADI6.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-PADI2 / PAD2 antibody [EPR26940-85] (<a href='/products/primary-antibodies/padi2-pad2-antibody-epr26940-85-ab322197'>ab322197</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human PADI2 expression vector containing a GFP-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human PADI1 expression vector containing a GFP-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human PADI3 expression vector containing a GFP-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a human PADI4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a human PADI6 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa,36 kDa,75 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-GFP antibody [E385] (AB32146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human NUAK1.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-NUAK2 antibody [EPR28627-717] (<a href='/products/primary-antibodies/nuak2-antibody-epr28627-717-ab322265'>ab322265</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human NUAK1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human NUAK2 expression vector containing a GFP-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 125 kDa,15 kDa,100 kDa

false

Exposure time: 3s

• WB

Unknown

Western blot - Anti-GFP antibody [E385] (AB32146)

All lanes:

Western blot - Anti-GFP antibody [E385] (ab32146) at 1/20000 dilution

Lane 1:

HeLa cells lysate (negative control)

Lane 2:

HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.1ng

Lane 3:

HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 0.5ng

Lane 4:

HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 1ng

Lane 5:

HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2ng

Lane 6:

HeLa cell lysate spike with recombinant Proprietary tag-GFP fusion protein at 2.5ng

Lane 7:

Proprietary tag-GFP 0.1mg/ml (positive control)

Predicted band size: 27 kDa

false

• WB

Supplier Data

Western blot - Anti-GFP antibody [E385] (AB32146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-GPCR GPRC5D antibody [EPR28376-41] (<a href='/products/primary-antibodies/gpcr-gprc5d-antibody-epr28376-41-ab315808'>ab315808</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a GFP-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human GPCR GPRC5D expression vector containing a a GFP-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33 kDa,15 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-GFP antibody [E385] (AB32146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands higher than 100 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-AKT (phospho T308) antibody [EPR26565-137] (ab324741) at 1/5000 dilution

Lane 1:

Untreated 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T treated with 100nM Calyculin A for 30min, whole cell lysate at 20 µg

Lane 3:

293T transfected with AKT1 (WT) expression vector containing a GFP-tag treated with 100nM Calyculin A for 30min, whole cell lysate at 20 µg

Lane 4:

293T transfected with AKT1 (T308A) expression vector containing a GFP-tag treated with 100nM Calyculin A for 30min, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60 kDa,36 kDa,86 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-GFP antibody [E385] (AB32146)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/10000 dilution.

In Western blot, Anti-TIM 4 antibody [EPR22304-3] (ab222093) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-HA tag antibody [RM1058] (<a href='/products/primary-antibodies/ha-tag-antibody-rm1058-ab314237'>ab314237</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-GFP-tag, whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

293T transfected with TIM 4 (WT) expression vector containing a HA-myc-His-GFP-tag and PDGFR TM domain, whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 78 kDa,36 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-GFP antibody [E385] (AB32146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Anti-GFP tag antibody (ab32146) staining at 1/1000 dilution. Exposure time : 10 seconds

All lanes:

Western blot - Anti-Vaccinia Virus antibody [EPR28236-80] (<a href='/products/primary-antibodies/vaccinia-virus-antibody-epr28236-80-ab312854'>ab312854</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a GFP tag whole cell lysate at 20 µg

Lane 2:

293T transfected with a Virion membrane protein A21 expression vector containi a GFP tag whole cell lysate at 20 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

Human kidney tissue lysate at 20 µg

Lane 6:

Mouse kidney tissue lysate at 20 µg

Lane 7:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 46 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-GFP antibody [E385] (AB32146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PADI1, PADI2, PADI3 and PADI6.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-PADI4 / PAD4 antibody [EPR26687-49] (<a href='/products/primary-antibodies/padi4-pad4-antibody-epr26687-49-ab315269'>ab315269</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human PADI4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human PADI1 expression vector containing a GFP-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human PADI3 expression vector containing a GFP-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a human PADI2 expression vector containing a GFP-tag, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a human PADI6 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-GFP antibody [E385] (AB32146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Smt3 antibody [EPR28614-578] (<a href='/products/primary-antibodies/smt3-antibody-epr28614-578-ab322402'>ab322402</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a GFP-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Saccharomyces cerevisiae SMT3 expression vector containing a GFP-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 16 kDa,37 kDa,36 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-GFP antibody [E385] (AB32146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 35kDa dilution.

All lanes:

Western blot - Anti-Rat Poly PR antibody [PERA534G] (<a href='/products/primary-antibodies/rat-poly-pr-antibody-pera534g-ab323367'>ab323367</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containi a EGPF-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a poly (PR)20 expression vector containi a EGFP-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/20000 dilution

Observed band size: 35 kDa,124 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-GFP antibody [E385] (AB32146)

In Western blot, Anti-GFP antibody [E385] (ab32146) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Cholera enterotoxin subunit B antibody [EPR27372-4] (<a href='/products/primary-antibodies/cholera-enterotoxin-subunit-b-antibody-epr27372-4-ab317738'>ab317738</a>) at 1/5000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a EGFP tag, whole cell lysate at 2 µg with NFDM/TBST

Lane 2:

293T transfected with a Cholera enterotoxin subunit B expression vector containing a EGFP tag, whole cell lysate 2 at 2 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa

false

Exposure time: 180s