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AB6556

Anti-GFP 抗体

Anti-GFP antibody

5

(61 Reviews)

|

(1504 Publications)

Anti-GFP antibody (ab6556) is a rabbit polyclonal antibody detecting GFP in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF, EM.

- Over 1310 publications
- Trusted since 2002

See more GFP antibodies and assay kits

別名を表示する

Green fluorescent protein, GFP

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB6556)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB6556)

This image shows a single primary hippocampal neuron from a primary culture overexpressing GFP stained with ab6556 at a dilution of 1/2000. This picture was kindly supplied as part of the review submitted by one of our customers.

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB6556)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GFP antibody (AB6556)

This image shows IF using GFP-expressing glial cells (green) transplanted into lesioned rat spinal cord. This was detected using ab6556 anti-GFP antibody and a FITC conjugated secondary antibody. Axons are labelled red by an antibody to neurofilament-200 and a rhodamine secondary. ab6556 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious. The top picture shows an optical section from a confocal microscope scan showing how a GFP cell wraps around a branched axon travelling longitudinally. The bottom picture consists of an optical section from another confocal scan showing a GFP cell enveloping an axon in the transverse plane. Review by Andrew Toft submitted 19 May 2004.

Electron Microscopy - Anti-GFP antibody (AB6556)
  • EM

Unknown

Electron Microscopy - Anti-GFP antibody (AB6556)

Specific labeling of a Trk-GFP fusion protein being synthesized on ER in sympathetic neurons infected with an adenovirus carrying the construct. The gold is associated with the ER membranes. This was done using a 1/5000 dilution of affinity purified antibody (ab6556). The tissue section was fixed and embedded using durcupan resin.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

アプリケーション

IP, IHC-P, EM, IHC-Fr, WB, ICC/IF, Flow Cyt

applications

免疫原

Recombinant Full Length Protein corresponding to Aequorea victoria GFP.

P42212

特異性

GFP antibody (ab6556) is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "EM" : {"fullname" : "Electron Microscopy", "shortname":"EM"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "EM-species-checked": "testedAndGuaranteed", "EM-species-dilution-info": "1/5000", "EM-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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製品の詳細

Anti-GFP antibody (ab6556) was first used in a scientific publication in 1999 and has been cited over 1310 times in peer reviewed journals. It's performance in Western Blot, IHC and immunofluorescence is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-GFP antibody (ab6556) has high sensitivity and specificity.

Anti-GFP antibody (ab6556) has 61 independent reviews from customers.

GFP antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-GFP antibody (ab6556) has been validated in EM, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP and WB.

Anti-GFP antibody (ab6556) specifically detects GFP (UniProt ID: P42212; Molecular weight: 27kDa) and is sold in 25 µL selling sizes.

Green Fluorescent Protein (GFP), originally derived from the jellyfish Aequorea victoria, emits a bright green fluorescence under ultraviolet or blue light. This unique property makes GFP an invaluable tool in molecular and cell biology research. Widely used to tag proteins, GFP allows scientists to visualize and track protein expression, localization and interactions within living cells. Key applications of GFP include fluorescence microscopy (ICC, IHC, IF), flow cytometry (FC) and western blotting (WB).
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
This antibody is an affinity purified rabbit anti-GFP antibody purified on an affinity chromatography column made with highly purified recombinant GFP.
バッファー組成
pH: 7.4 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GFP also known as Green Fluorescent Protein acts as a bioluminescent marker derived from the jellyfish Aequorea victoria. GFP is popular in molecular biology for its fluorescence properties making it useful for visualizing proteins. This protein has a molecular weight of approximately 27 kDa. Researchers and scientists often express GFP in various organisms as a luminescent tag helping them observe protein expression localization and interaction within cells. GFP tagging involves the fusion of GFP to a protein of interest enabling the study of the protein's function and dynamics without affecting the host cell.
Biological function summary

GFP serves as a marker due to its ability to emit green fluorescence without requiring additional substrates or cofactors. GFP does not function within complexes like other proteins but acts as a standalone tool to monitor physiological processes. Scientists utilize techniques such as Western blot ELISA and microscopy along with GFP to track and quantify proteins inside living cells. Anti-GFP antibodies can detect GFP fusion proteins in various applications providing valuable insights into protein behavior and allowing robust assays involving GFP.

Pathways

GFP itself does not participate actively in traditional biochemical or signaling pathways. Instead it enables visual tracking within pathways. Researchers utilize GFP to study pathways like MAPK/ERK and PI3K/AKT where they track proteins related to these pathways using GFP tagging. For instance fusing GFP with proteins like ERK1/2 allows tracking phosphorylation events and signal transduction in living cells leading to better understanding of cellular responses to different stimuli.

Researchers use GFP as a model to study gene expression and protein interactions under disease conditions. For example in neurological disorders GFP helps visualize neuronal pathways and protein aggregation processes. By tagging proteins such as amyloid precursor protein (APP) or tau with GFP scientists can study their role in Alzheimer's disease progression. Similarly GFP facilitates the investigation of cancer pathways allowing visualization of tumor-related proteins and helping researchers study how cancer cells grow and invade tissues supporting cancer research and therapy development.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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文献 (1504)

Recent publications for all applications. Explore the full list and refine your search

Nature protocols : PubMed40940523

2025

Labeling, isolation and characterization of cell-type-specific exosomes derived from mouse skin tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Yadav,Anu Sharma,Mohini Moulick,Parmeshwar V Gavande,Aparajita Nandy,Yi Xuan,Chandan K Sen,Subhadip Ghatak

Viruses 17: PubMed40872789

2025

The Replication Function of Rabies Virus P Protein Is Regulated by a Novel Phosphorylation Site in the N-Terminal N Protein-Binding Region.

Applications

Unspecified application

Species

Unspecified reactive species

Ericka Tudhope,Camilla M Donnelly,Ashish Sethi,Cassandra David,Nicholas Williamson,Murray Stewart,Jade K Forwood,Paul R Gooley,Gregory W Moseley

Science advances 11:eadw9153 PubMed40864707

2025

Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote.

Applications

Unspecified application

Species

Unspecified reactive species

Jason Banda,Shraboni Ghosh,Dipan Roy,Kishor D Ingole,Lisa Clark,Eshan Sharma,Sumesh Kakkunath,Kawinnat Sue-Ob,Rahul Bhosale,Leah Band,Srayan Ghosh,Darren Wells,Jonathan Atkinson,Nicholas J Provart,Malcolm J Bennett,Kathryn S Lilley,Andrew Jones,Miguel De Lucas,Anthony Bishopp,Ari Sadanandom

Endocrinology 166: PubMed40874857

2025

Harnessing Distinct Tissue-Resident Immune Niches via S100A9/TLR4 Improves Ketone, Lipid, and Glucose Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Lucibello,Gloria Ursino,Pryscila D S Teixeira,Szabolcs Zahoran,Francesca Fanuele,Marinos Kallikourdis,Florian Visentin,Christelle Veyrat-Durebex,Ariane Widmer,Yibo Wu,Marco Cremonesi,Claes B Wollheim,Perrine Castets,Giorgio Ramadori,Roberto Coppari

Scientific reports 15:29772 PubMed40804104

2025

A fluorescence lifetime-based FLIM-timer for measuring the protein turnover of transcription factor Nrf2 in live cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dina Dikovskaya,Claudia Bento-Pereira,Kanade Shiga,Andrea Corno,Maureen Higgins,Rachel Toth,Adrian T Saurin,Albena T Dinkova-Kostova

Lab animal 54:228-237 PubMed40804162

2025

Cell type-specific in vivo proteomes with a multicopy mutant methionyl tRNA synthetase mouse line.

Applications

Unspecified application

Species

Unspecified reactive species

Rodrigo Alvarez-Pardo,Susanne Tom Dieck,Kristina Desch,Belquis Nassim Assir,Cristina Olmedo Salinas,Riya S Sivakumar,Julian D Langer,Erin M Schuman,Beatriz Alvarez-Castelao

Science advances 11:eadv2902 PubMed40749059

2025

Glia-derived noncanonical fatty acid binding protein modulates brain lipid storage and clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yin,Hsueh-Ling Chen,Anna Grigsby-Brown,Yi He,Myriam L Cotten,Jacob Short,Aidan Dermady,Jingce Lei,Mary Gibbs,Ethan S Cheng,Dean Zhang,Caixia Long,Jennifer Tabet,Lele Xu,Tiffany Zhong,Rinat Abzalimov,Mariam Haider,Rong Sun,Ye He,Qiangjun Zhou,Nico Tjandra,Quan Yuan

iScience 28:113081 PubMed40740498

2025

Desmosomal cadherin tension loss in pemphigus vulgaris mediated by the inhibition of active RhoA at cell-cell adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Jin,Jordan Rosenbohm,Eunju Kim,Bahareh Tajvidi Safa,Amir Ostadi Moghaddam,Kristina Seiffert-Sinha,Merced Leiker,Elijah Jones,Haiwei Zhai,Sindora R Baddam,Grayson Minnick,Yucheng Huo,James K Wahl,Fanben Meng,Changjin Huang,Jung Yul Lim,Daniel E Conway,Animesh A Sinha,Ruiguo Yang

Nature communications 16:6734 PubMed40695820

2025

Age mosaic of gut epithelial cells prevents aging.

Applications

Unspecified application

Species

Unspecified reactive species

Peizhong Qin,Qi Wang,You Wu,Qiqi You,Mingyu Li,Zheng Guo

Neurobiology of stress 37:100743 PubMed40686530

2025

Chronic stress and cytogenesis ablation disrupt hippocampal neuron connectivity, with fluoxetine restoring function with sex-specific effects.

Applications

Unspecified application

Species

Unspecified reactive species

Inês Ribeiro,Tiago Silveira-Rosa,Joana Martins-Macedo,Luísa Marques-Ferraz,Ana Rita Dourado,Gonçalo Martins-Ferreira,Fanny Farrugia,Ana João Rodrigues,Djoher Nora Abrous,Nuno Dinis Alves,Patrícia Patrício,Luisa Pinto
View all publications
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組合せ製品

Secondary Antibodies

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Primary Antibodies

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BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Abcam product promise

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