Anti-GFAP 抗体 [ZRF-1] (ab154474)
![Western blot - Anti-GFAP antibody [ZRF-1] (ab154474)](https://www.abcam.co.jp/ps/products/154/ab154474/Images/ab154474-160654-anti-gfap-antibody-zrf-1-western-blot.jpg "Western blot - Anti-GFAP antibody [ZRF-1] (ab154474)")
Key features and details
- Mouse monoclonal [ZRF-1] to GFAP
- Suitable for: WB, IP
- Reacts with: Human, Zebrafish
- Isotype: IgG1
製品の概要
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製品名
Anti-GFAP antibody [ZRF-1]
GFAP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [ZRF-1] to GFAP -
由来種
Mouse -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human, Zebrafish
非交差種: Mouse, Rat -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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ポジティブ･コントロール
- Human brain and Zebrafish brain homogenates.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
特記事項（精製）
Near homogeneity as judged by SDS-PAGE. ab154474 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
ZRF-1 -
アイソタイプ
IgG1 -
軽鎖の種類
lambda -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154474の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa.
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| IP |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 51 kDa. |
|
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
組織特異性
Expressed in cells lacking fibronectin. -
関連疾患
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylated by PKN1. -
細胞内局在
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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参照データベース
- Entrez Gene: 2670 Human
- Entrez Gene: 30646 Zebrafish
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: Q58EE9 Zebrafish
- Unigene: 514227 Human
- Unigene: 32667 Zebrafish
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別名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
画像
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Western blot - Anti-GFAP antibody [ZRF-1] (ab154474)All lanes : Anti-GFAP antibody [ZRF-1] (ab154474) at 0.5 µg/ml
Lane 1 : Human Brain Homogenate lysate
Lane 2 : Zebrafish Brain Homogenate lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution
Predicted band size: 51 kDa -
![Immunoprecipitation - Anti-GFAP antibody [ZRF-1] (ab154474)](https://www.abcam.co.jp/ps/products/154/ab154474/Images/ab154474-160653-anti-gfap-antibody-zrf-1-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GFAP antibody [ZRF-1] (ab154474)Immunoprecipitation Western Blot for ab154474.
All lanes: ab154474 at 0.5 µg/ml.
Lane 1: Zebrafish Brain Homogenate lysate 10 µg
Lane 2: GFAP immunoprecipitated from 1 mg Zebrafish Brain Homogenate lysate 25µl.
Secondary: Goat polyclonal to Mouse IgG - AP at 1/3000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab154474 は 6 報の論文で使用されています。
- Hjort M et al. In situ assembly of bioresorbable organic bioelectronics in the brain. Nat Commun 14:4453 (2023). PubMed: 37488105
- Ceriani R & Whitlock KE Gonadotropin Releasing Hormone (Gnrh) Triggers Neurogenesis in the Hypothalamus of Adult Zebrafish. Int J Mol Sci 22:N/A (2021). PubMed: 34072957
- Demirci Y et al. Comparative Transcriptome Analysis of the Regenerating Zebrafish Telencephalon Unravels a Resource With Key Pathways During Two Early Stages and Activation of Wnt/ß-Catenin Signaling at the Early Wound Healing Stage. Front Cell Dev Biol 8:584604 (2020). PubMed: 33163496
- McCallum S et al. Enteric glia as a source of neural progenitors in adult zebrafish. Elife 9:N/A (2020). PubMed: 32851974
- Luo JJ et al. CRISPR/Cas9-based genome engineering of zebrafish using a seamless integration strategy. FASEB J 32:5132-5142 (2018). PubMed: 29812974
- Yang Q et al. Serotonin Activated Hepatic Stellate Cells Contribute to Sex Disparity in Hepatocellular Carcinoma. Cell Mol Gastroenterol Hepatol 3:484-499 (2017). IF ; Zebrafish . PubMed: 28462385