Anti-GFAP 抗体 [2A5] (ab4648)
Key features and details
- Mouse monoclonal [2A5] to GFAP
- Suitable for: IHC-FrFl, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GFAP antibody [2A5]
GFAP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2A5] to GFAP -
由来種
Mouse -
特異性
This clone gives a much stronger signal in pig, and human samples than in rodents. For rodent (mouse, rat, etc) samples use ab68428.
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アプリケーション
適用あり: IHC-FrFl, WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human, Pig -
免疫原
Full length native protein (purified) corresponding to Pig GFAP. A preparation of purified pig spinal cord GFAP.
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ポジティブ・コントロール
- IHC-P: Human brain tissue, human cerebellum tissue. IHC-FreeFloat: Rat cerebellum tissue: WB: Pig brain tissue lysate; Rat spinal cord tissue lysate; Mouse spinal cord tissue lysate. ICC: Rat neuron/glia cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant
Concentrated Tissue Culture Supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Antibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
2A5 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4648の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-FrFl |
1/500.
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WB | (1) |
1/2000. Predicted molecular weight: 49 kDa.
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IHC-P | (3) |
1/500.
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ICC/IF | (5) |
1/10 - 1/50.
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特記事項 |
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IHC-FrFl
1/500. |
WB
1/2000. Predicted molecular weight: 49 kDa. |
IHC-P
1/500. |
ICC/IF
1/10 - 1/50. |
ターゲット情報
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機能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
組織特異性
Expressed in cells lacking fibronectin. -
関連疾患
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylated by PKN1. -
細胞内局在
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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参照データベース
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 396562 Pig
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- SwissProt: P47819 Rat
see all -
別名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)
Immunohistochemistry analysis of paraffin-embedded human cerebellum tissue sections labelling GFAP with ab4648 at 1/500 dilution (1 mg/mL). Sections were stained with ab4648 using the HRP/DAB staining. Sections were counterstained with hematoxylin/eosin. Antigen retrieval was heat mediated using Antigen retrieval buffer (100X citrate buffer) (pH 6.0) (ab93678) for 15 minutes.
To the right is a region of cerebellar molecular layer containing the prominent cytoskeletal fibers of Bergmann glia which are strongly positive for GFAP. The middle shows a region of the granular layer and to the left is white matter, both of which contain GFAP positive astrocytes.The immunostaining was performed with the Vector ImmPress rat adsorbed horse anti-mouse IgG detection kit. -
Immunofluorescence analysis of rat cerebellum tissue labeling GFAP with ab4648 at 1/500 dilution (Red), Costained with parvalbumin antibody at 1/2,000 dilution (Green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and free-floating 45μM sections were stained with above antibodies. ab4648 stains the processes of Bergmann glia and astrocytes. The Pvalb antibody labels perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The staining on rodent tissues is specific but not as robust as on human material.
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Lanes 2-8 : Anti-GFAP antibody [2A5] (ab4648) at 1/2000 dilution
Lane 1 : MW markers
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
Lane 6 : Pig brain tissue lysate
Lane 7 : Recombinant rat GFAP protein
Lane 8 : Recombinant human GFAP protein
Predicted band size: 49 kDa -
Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab 4648 (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [2A5] (ab4648)Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
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Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
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Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (102)
ab4648 は 102 報の論文で使用されています。
- Lee HJ et al. Potential Risk of Choline Alfoscerate on Isoflurane-Induced Toxicity in Primary Human Astrocytes. J Korean Neurosurg Soc 67:418-430 (2024). PubMed: 37859347
- Liang T et al. HSP90β regulates EAAT2 expression and participates in ischemia‑reperfusion injury in rats. Mol Med Rep 29:N/A (2024). PubMed: 37975223
- Aidos L et al. Intestine Health and Barrier Function in Fattening Rabbits Fed Bovine Colostrum. Vet Sci 10:N/A (2023). PubMed: 37999480
- Shi T et al. Acupuncture Relieves Cervical Spondylosis Radiculopathy by Regulating Spinal Microglia Activation Through MAPK Signaling Pathway in Rats. J Pain Res 16:3945-3960 (2023). PubMed: 38026466
- Geng L et al. MLKL deficiency alleviates neuroinflammation and motor deficits in the α-synuclein transgenic mouse model of Parkinson's disease. Mol Neurodegener 18:94 (2023). PubMed: 38041169