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リコンビナントRabMAb

Anti-Germinal Center Kinase/GCK 抗体 [EPR14304] (ab184169)

Submit a review Submit a question References (2)
Western blot - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
  • Flow Cytometry (Intracellular) - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
  • Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14304] to Germinal Center Kinase/GCK
  • Suitable for: IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Germinal Center Kinase/GCK antibody [EPR14304]
    Germinal Center Kinase/GCK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14304] to Germinal Center Kinase/GCK

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Namalwa, Ramos and Human fetal brain lysates; Human hepatocellular carcinoma tissue; Ramos cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184169の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 92 kDa).
Flow Cyt (Intra)
1/170.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 92 kDa).
Flow Cyt (Intra)
1/170.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. May play a role in the regulation of vesicle targeting or fusion.

  • 組織特異性

    Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.

  • 配列類似性

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.

  • 細胞内局在

    Cytoplasm. Basolateral cell membrane. Golgi apparatus membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B lymphocyte serine/threonine protein kinase antibody
      • B lymphocyte serine/threonine-protein kinase antibody
      • BL44 antibody
      • GC kinase antibody
      • GCK antibody
      • Germinal center kinase antibody
      • germinal centre kinase (GC kinase) antibody
      • M4K2_HUMAN antibody
      • Map4k2 antibody
      • MAPK/ERK kinase kinase kinase 2 antibody
      • MEK kinase kinase 2 antibody
      • MEKKK 2 antibody
      • Mitogen activated protein kinase kinase kinase kinase 2 antibody
      • Mitogen-activated protein kinase kinase kinase kinase 2 antibody
      • Rab8 interacting protein antibody
      • Rab8 interacting protein, formerly antibody
      • Rab8-interacting protein antibody
      • RAB8IP antibody
      • RAB8IP, formerly antibody
      see all

    画像

    • Western blot - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
      Western blot - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
      All lanes : Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169) at 1/10000 dilution

      Lane 1 : Namalwa cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : Human fetal brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 92 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma labeling Germinal Center Kinase/GCK with ab184169 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
      Flow Cytometry (Intracellular) - Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)

      Intracellular flow cytometric analysis of Ramos cells (2% paraformaldehyde-fixed) labeling Germinal Center Kinase/GCK with ab184169 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

       

       

    • Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)
      Anti-Germinal Center Kinase/GCK antibody [EPR14304] (ab184169)

    参考文献 (2)

    ab184169 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184169 は 2 報の論文で使用されています。

    • Zhang R  et al. An NF-κB/OVOL2 circuit regulates glucose import and cell survival in non-small cell lung cancer. Cell Commun Signal 20:40 (2022). PubMed: 35346238
    • Ho CM  et al. Inhibition of IKKa by BAY61-3606 Reveals IKKa-Dependent Histone H3 Phosphorylation in Human Cytomegalovirus Infected Cells. PLoS One 11:e0150339 (2016). WB . PubMed: 26930276

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